Serum response factor promotes axon regeneration following spinal cord transection injury
Guo-Ying Feng1
Nai-Li Zhang1
Xiao-Wei Liu1
Ling-Xi Tong1
Chun-Lei Zhang1
Shuai Zhou1
Lu-Ping Zhang1
Fei Huang2
1.Institute of Neurobiology,Binzhou Medical University,Laishan,Shandong Province,China2.Institute of Neurobiology,Binzhou Medical University,Laishan,Shandong Province,China;Schoolof Health and Life Sciences,University of Health and Rehabilitation Sciences,Qingdao,Shandong Province,China
摘要：Studies have shown that serum response factor is beneficialfor axonal regeneration of peripheral nerves.However,its role after central nervous system injury remains unclear.In this study,we established a rat model of T9-T10 spinal cord transection injury.We found that the expression of serum response factor in injured spinal cord gray matter neurons gradually increased with time,reached its peak on the 7th day,and then gradually decreased.To investigate the role of serum response factor,we used lentivirus vectors to overexpress and silence serum response factor in spinal cord tissue.We found that overexpression of serum response factor promoted motor function recovery in rats with spinal cord injury.Qualitative observation of biotinylated dextran amine anterograde tracing showed that overexpression of serum response factor increased nerve fibers in the injured spinal cord.Additionally,transmission electron microscopy showed that axon and myelin sheath morphology was restored.Silencing serum response factor had the opposite effects of overexpression.These findings suggest that serum response factor plays a role in the recovery of motor function after spinal cord injury.The underlying mechanism may be related to the regulation of axonal regeneration.
机标关键词：regenerationcordaxonfactorfollowinginjurypromotesresponse
论文发表日期：2023-09-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 1956-1960 )
英文信息