Single-cell and spatial omics:exploring hypothalamic heterogeneity
Muhammad Junaid1
Eun Jeong Lee2
Su Bin Lim1
1.Department of Biochemistry & Molecular Biology,Ajou University School of Medicine,Suwon,South Korea;Department of Biomedical Sciences,Graduate School of Ajou University,Suwon,South Korea2.Department of Biomedical Sciences,Graduate School of Ajou University,Suwon,South Korea;Department of Brain Science,Ajou University School of Medicine,Suwon,South Korea
摘要：Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions.Over the past decade,single-cell and spatial omics technologies have significantly evolved,overcoming initial technical challenges in capturing and analyzing individual cells.These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus.Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus.However,single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue,potentially resulting in the loss of spatial information concerning neuronal networks.Spatial transcriptomics methods,by bypassing the cell dissociation,can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies.In this review,we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types,driven by recent high-throughput achievements.
机标关键词：spatialexploringheterogeneityhypothalamicomicssingle-cell
论文发表日期：2025-06-27
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：16( 1525-1540 )
英文信息