Effects of Tensile Stimulation and Electrical Stimulation on the Connexion-43 mRNA Levels of Cardiomyocytes in vitro
LI Ping
LI Yu
LIU Li-zhen
JIA Lu
SONG Yan-hui
JIA Xiao-ling
FAN Yu-bo
摘要：The heart is regulated by excitation-contraction mechanism and electrical stimulation, so both cardiac tissues and myocytes in vivo are affected by the mechanical and electrical factors at the same time. The device that can supply both tensile stimulation and electrical stimulation was produced. Cardiomyocytes were isolated by trypsin and collagenase Ⅱ combined digestion. After 72 h's culture, different electrical stimulations (0.5 V/cm, 1 V/cm, 2 V/cm;1 Hz;2 ms;30 min) and mechanical stretching force (tensile 10％, 6 h) were used to stimulate cultured cardiomyocytes respectively. Then the combinative stimulation of mechanical and electrical stimulation with 0.5 V/cm was used to apply to cardiomyocytes simultaneously. After stimulation, Connexin-43 (Cx-43) mRNA level was detected by RT-PCR. The stimulation by electrical pulse of 0.5 V/cm resulted in obvious enhancement of Cx-43 mRNA level compared to the control group and the Cx-43 mRNA levels at 0.5 V/cm were higher significantly than those under both 1.0 V/cm and 2.0 V/cm. After 10％ of tensile stimulation for 6 h, expressions of Cx-43 mRNA were also significantly promoted. The combinative effect of mechanical and electrical stimulation on the expression of Cx-43 mRNA is higher than that under unique tensile stimulation or electrical stimulation, which is more beneficial to the development and connection of cardiomyocytes.
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论文发表日期：2018-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 126-131 )
英文信息