Role of miRNA-27a in Regulating the Immune Function of Pulmonary Tuberculosis Rat Model

XU Bai-lan

摘要：Objective: To analyze the role of miRNA-27a in regulating the immune function of the pulmonary tuberculosis rat model. Methods:40 healthy female rats were divided into 4 groups: healthy control group, model group, silence group, and overexpression group, with 10 rats in each group. The control group was not treated , and the other three groups of rats were injected with 200 μL of Mycobacterium bacilli suspension through the tail vein to establish a rat model of pulmonary tuberculosis, in which the model group was not treated, and the silence group was injected with 10 μL of miRNA-27 inhibition vector lentiviral suspension, and the overexpression group was injected with 10μL of miRNA-27 expression vector lentiviral suspension. The expression level of miRNA-27a, peripheral blood T lymphocyte subsets, thymus index, inflammatory index, and TLR4/NF-κB signal pathway-related protein level in the four groups of rats were compared and analyzed. Results: Compared with the healthy control group, the expression levels of the silence group and model group were significantly decreased, while the expression level of the overexpression group was significantly increased; Compared with the model group and addiction group, the expression level of the overexpression group was significantly increased, with significant differences( P<0.05). Compared with the healthy control group, CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, and IκB in the model group, silence group, and overexpression group were significantly higher than those in the control group;The levels of CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, IκB decreased significantly, with significant differences ( P<0.05); Compared with the overexpression group, CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB levels were significantly lower; The levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB levels were significantly increased, with significant differences ( P<0.05). Compared with the silence group, the levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB were significantly increased and the levels of CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB were significantly lower in the overexpression group, with significant differences (P<0.05). Conclusion:Overexpression of miRNA-27a can inhibit the TLR4/NF-κB signal pathway and regulate the immune function of rats with pulmonary tuberculosis, which in turn reduce lung injury in rats.