Effect of Atorvastatin on the Expression of Inflammatory Cytokines in Rats with Klebsiella Pneumonia

QUE Wei-wei; LI You-tang; WENG Duan-li

摘要：Objective: To explore the effect of atorvastatin on the expression of inflammatory cytokines in rats with Klebsiella pneumonia. Methods: 90 healthy SPF-grade SD rats were randomly divided into three groups: atorvastatin group, model group and blank group (with 10 rats in each group), 30 rats in each treatment period (3,6,9 d). A rat model of Klebsiella pneumonia was constructed, in which the blank group and the model group were given the same volume of saline, while the atorvastatin group was given 10 ml/kg of atorvastatin by intraperitoneal instillation. The rats were killed on the 10th day after administration, and the lung tissue was extracted to detect the pathological results and the expression of inflammatory cytokines was detected in serum. Results: Lung histopathology showed that lung histopathology and fibrosis were improved in atorvastatin group, and alveolar structure integrity≥50％and collagen fiber precipitation≤10％in atorvastatin group indicated that the model was successful. The expression of inflammatory cytokines showed that the levels of IL-6, TNF-α and TGF-β in the atorvastatin group and the model group were significantly increased compared with the blank group, with statistically significant differences ( P＜0.05). The levels of IL-6, TNF-α and TGF-β in atorvastatin group were lower than those in model group, and the levels of IL-10 in atorvastatin group were higher than those in model group, with statistically significant differences (P＜0.05). After 9 days, the levels of IL-6, TNF-α, TGF-β and IL-10 in the atorvastatin group and the model group were higher than those in the blank group, with statistically significant differences ( P＜0.05). The results of immunological function study showed that WBC, RBC and PLT in orvastatin group and model group were significantly reduced at 3 d compared with the blank group, with statistically significant differences (P＜0.05). After 6 and 9 d, WBC, RBC and PLT in atorvastatin group and model group were lower than those in blank group, and WBC, RBC and PLT in atorvastatin group were lower than those in model group, with statistically significant differences (P＜0.05). Conclusion: Atorvastatin can significantly improve the immune dysfunction and the expression of inflammatory cytokines in rats with Klebsiella pneumonia.