Detection of IgM and IgG Antibodies in Mice with Hepatitis C Virus by Magnetic Particle Chemiluminescence Method
XU You-qiang1
JIA Quan-sheng1
ZHOU Ting-ting2
LU Fang2
YANG Jing3
1.Xinjiang Kingmed for Clinical Laboratory Co.,Ltd.,Urumqi Xinjiang 830000,China2.Jilin Kingmed for Clinical Laboratory Co.,Ltd.,Changchun Jilin 130000,China3.Jilin Agricultural University,Changchun Jilin 130000,China
摘要：Objective:To explore the application value of magnetic particle chemiluminescence method in the detection of hepatitis C virus(HCV)IgM,IgG and other antibody indicators in mice.Methods:A total of 90 mice with HCV were selected as the research objects.All the mice were tested for HCV IgM and IgA antibody,and then their venous blood samples were taken to detect the antibody index by enzyme-linked immunosorbent assay,magnetic particle chemiluminescence method and indirect agglutination method,and the results and positive rates of the three groups were compared.Results:There was no significant difference in the positive rate among the three groups(P＞0.05).The specificity,accuracy and sensitivity of indirect agglutination test for IgM were 90.79％,80.00％and 30.00％,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 97.44％,93.33％and 80.00％,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were significantly lower than those of magnetic particle chemiluminescence assay(P＜0.05).For IgA,the specificity,accuracy and sensitivity of indirect agglutination assay were 90.67％,78.66％and 20.00％,respectively.The specificity,accuracy and sensitivity of magnetic particle chemiluminescence assay were 96.25％,92.35％and 80.00％,respectively.The specificity,accuracy and sensitivity of indirect agglutination assay were lower than those of magnetic particle chemiluminescence assay,and the difference was statistically significant(P＜0.05).Conclusion:The magnetic particle chemiluminescence method is more effective in the detection of IgM,IgG and other antibodies in mice with HCV.
机标关键词：particledetectionmethodviruschemmicewithantibodies
分类号：R373.1(医学微生物学（病原细菌学、病原微生物学）)
论文发表日期：2023-12-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 177-184 )
英文信息