The Effect of Serum Deprivation Response-related Protein on Hepatocellular Carcinoma Cells via the ASK1-JNK/p38 MAPK Signaling Pathway
LIN Yan-hui
Fujian Provincial Hospital,Fuzhou Fujian 350001,China
摘要：Objective:To investigate the effect of serum deprivation response-related protein(SDPR)on hepatocellular carcinoma(HCC)cells through the ASK1-JNK/p38 MAPK signaling pathway.Methods:A total of 30 HCC tissue samples and adjacent non-tumor tissues were collected from HCC patients who underwent hepatectomy in our hospital from June 2023 and June 2024.Quantitative real-time PCR and Western blot were used to measure the expression levels of SDPR,ASK1,JNK,and p38 MAPK in HCC tissues.Cell proliferation,migration,and apoptosis were compared between SDPR-low and SDPR-high HCC tissues.Results:SDPR mRNA levels and SDPR/GAPDH ratios in HCC tissues were significantly lower than those in adjacent tissues(P<0.05).The OD values of SDPR-low HCC tissues on Days 3,4,and 5 were significantly higher than those of SDPR-high HCC tissues(P<0.05).The number of migrated cells at 20 and 40 h in SDPR-low HCC tissues was significantly greater than in SDPR-high tissues,while the apoptosis rate was significantly lower(P<0.05).SDPR-low HCC tissues exhibited significantly reduced mRNA and protein expression levels of ASK1,JNK,and p38 MAPK compared to SDPR-high tissues(P<0.05).Conclusion:SDPR is downregulated in HCC tissues.Low SDPR expression promotes HCC cell proliferation and migration while inhibiting apoptosis,potentially through modulating the expression of proteins in the ASK1-JNK/p38 MAPK signaling pathway.
机标关键词：pathwayproteinmapkcellscarcinomadeprivationeffecthepatocellular
分类号：R735.7(消化系肿瘤)
论文发表日期：2024-12-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 136-142 )
英文信息