Experimental study on Inhibition of Autophagy in Triple-negative Breast Cancer Mice by Microbubble-enhanced Irradiation with Low-frequency Ultrasound
SHAO Jian-bin
FANG Yin
The First Affiliated Hospital of Xiamen University,School of Medicine,Xiamen University,Xiamen Fujian 361000,China
摘要：Objective:To investigate the role of low-frequency ultrasound(LFUS)combined with microbubble enhancement in inhibiting autophagy in a mouse model of triple-negative breast cancer(TNBC).Methods:A total of 32 SPF-grade BALB/C mice were randomly divided into four groups:control group,TNBC group,LFUS group,and LFUS+M group.The mice in each group were treated with tail vein injection of normal saline,establishment of TNBC model via tumor xenotransplantation+normal saline in-jection,LFUS irradiation,and sulfur hexafluoride microbubbles(SonoVue)+LFUS irra-diation,respectively.The tumor growth curves and survival status of TNBC in the four groups of mice were monitored.Western blotting was used to determine the expression level of autophagy-related proteins,and MTT assay and flow cytometry were used to de-tect the proliferation of mammary tumor cells in each group.Results:Compared with the control group,the tumor volume of mice in the LFUS group and LFUS+M group was sig-nificantly reduced.The tumor growth curve of the LFUS group slowed down compared with that of the TNBC group,while the tumor volume of the LFUS+M group decreased significantly on the 4th day,and the tumor tissue growth trend was slower than that of the TNBC group and the LFUS group,and the survival time was prolonged.The Bcl-2 and Bax levels of breast tumor histiocytes in the LFUS+M group were lower than those in the other three groups,Beclin1 was higher than those in the control group and TNBC group,and LC3II./LC3I was higher than that in the control group and the other two groups(P<0.05).Conclusion:Low-frequency ultrasound microbubble-enhanced irradiation can significantly inhibit the tumor volume growth in TNBC mice,and inhibit their prolif-erative activity by promoting the autophagy ability of tumor cells,with particularly obvi-ous effects on Bcl-2 and Beclin1,prolonging the survival time of mice.
机标关键词：inhibitionultrasoundcancerstudywithmiceautophagybreast
分类号：R737.9(泌尿生殖器肿瘤)
论文发表日期：2025-03-31
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 34-39 )
英文信息