Construction of Prokaryotic Expression Vector for pbv220/NT4-ADNF-9
ZHENG Guo-xi
ZHU Kang
JING Yang
WEI Jun-rong
ZHU Hong-liang
摘要：Objective To construct a prokaryotic expression vector bearing fusion gene NT4-ADNF-9 for future studies on genetic therapies for sensorineural deafness. Methods Double strand ADNF-9 Edna was synthesized using asymmetrical primer/templates and ligated to the 3' terminal of signal and leader peptides of neurotrophin 4 (NT4). The fusion gene NT4-ADNF-9, was subeloned into prokaryotic expression vector Pbv220, and named Pbv220/NT4-ADNF-9. DNA sequence of the fusion gene was analyzed. The fusion protein was isolated by SDS-PAGE and its bioactivity was evaluated using primary culture of day 8 chicken embryonic DRGcells. Results The correct sequence of fusion gene NT4-ADNF-9 was successfully subcloned into the Pbv220 vector. The expressed ADNF-9 protein showed its effects in promoting cell survival and neurite growth. Conclusion Prokaryotic expression vector Pbv220/NT4-ADNF-9 was constructed successfully and the expressed fusion protein demonstrated satisfactory bioactivity.
机标关键词：expression vectorfusion genefusion proteinsequence ofNT4primary culturecell survivalSDS-PAGE
论文发表日期：2008-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 103-107 )
英文信息