THE COCHlEAR RIBBON SYNAPTIC RESPONSE TO AMINOGIYCOSIDE OTOTOXICITY IN C57BL/6J MICE
LIU Ke
ZHAO Ning
SHI Chuang
WU Nan
LIU Huizhan
ZHANG Yue
YANG Weiyan
YANG Shiming
摘要：Objective To investigate the early change of cochlear ribbon synapses on inner hair cells in response to aminoglycoside ototoxicity. Methods C57BL/6J mice received intraperitoneal injection of gentamicin (100 mg/kg/day), and the apical coil organ of Corti was examined on the 4th, 7th and 10th day (n=10). Litter-mates without gentamicin treatment served as controls (n=10). RIBEYE on the presynaptic membrane and AMPA receptors on the postsynaptic membrane were labeled with CtBP2 or GluR2/3 respectively. Three di-mension reconstruction was conducted using the 3DS MAX 8.0 software. Results There were no disruptions of outer or inner hair cells in all groups. However, the number of ribbon synapses on cochlear inner hair cells increased significantly within 7 days after gentamicin exposure (P<0.01), followed by a significant de-crease after 7 days.Conclusion During the early stage of aminoglycoside ototoxicity, increased population of cochlear ribbon synapses may indicate a significant down-regulation of synaptic function.
机标关键词：hair cellsintraperitoneal injectionAMPA receptors
资助基金：Be-ijing Natural Science Foundation (5122040)Additional-ly,this work was supported by grants from the China Post-doctoral Science Foundation(201003779)Additional-ly,this work was supported by grants from the China Post-doctoral Science Foundation(20100470103)the National Natural Science Foundation of China (NSFC)(31040038)
论文发表日期：2014-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 16-20 )
英文信息