Transcript variants and expression profiles analysis of Mitf gene in minipigs
Weiwei Guo
Lili Ren
Lei Chen
Yu Ning
Lidong Zhao
Shiming Yang
摘要：Object:To identify transcript variants and expression patterns of porcine Mitf. Materials and methods:A pairwise BLAST search at NCBI database was performed to deduce the structure of porcine Mitf gene. Subsequently, 50 RACE and fluorescent quantitative RT-PCR were used to analyze the expression pattern of porcine Mitf in different tissues. Results:Four transcript variants of porcine Mitf, MITF-A, MITF-H, MITF-M and MITF-SUS were identified, all sharing high homology with those in humans, except Mitf-SUS. Conclusion:The sequence of porcine Mitf appear highly homologous to human MITF. However, only 4 transcript variants of porcine Mitf were identified in these minipigs, less than the 9 transcript variants in human MITF.
机标关键词：expression patternsequence of
资助基金：grants from the Major State Basic Research Development Program of China (973 )grants from the Major State Basic Research Development Program of China (Program)(#2011CBA01000)the National Basic Research Program of China (973 Program)(#2012CB967900)the National Natural Science Foundation of China(81400472)
论文发表日期：2015-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 83-86 )
英文信息