Antioxidative stress-induced damage in cochlear explants
Dalian Ding
Jianghui Zhang
Fang Liu
Peng Li
Weidong Qi
Yazhi Xing
Haibo Shi
Haiyan Jiang
Hong Sun
Shankai Yin
Richard Salvi
摘要：The imbalance of reactive oxygen species and antioxidants is considered to be an important factor in the cellular injury of the inner ear. At present, great attention has been placed on oxidative stress. However, little is known about fighting oxidative stress. In the current study, we evaluated antioxidant-induced cochlear damage by applying several different additional antioxidants. To determine whether exces-sive antioxidants can cause damage to cochlear cells, we treated cochlear explants with 50μM M40403, a superoxide dismutase mimetic, 50μM coenzyme Q-10, a vitamin-like antioxidant, or 50μM d-methio-nine, an essential amino acid and the important antioxidant glutathione for 48 h. Control cochlear ex-plants without the antioxidant treatment maintained their normal structures after incubation in the standard serum-free medium for 48 h, indicating the maintenance of the inherent oxidative and anti-oxidant balance in these cochlear explants. In contrast, M40403 and coenzyme Q-10-treated cochlear explants displayed significant hair cell damage together with slight damage to the auditory nerve fibers. Moreover, d-methiodine-treated explants exhibited severe damage to the surface structure of hair cells and the complete loss of the spiral ganglion neurons and their peripheral fibers. These results indicate that excessive antioxidants are detrimental to cochlear cells, suggesting that inappropriate dosages of antioxidant treatments can interrupt the balance of the inherent oxidative and antioxidant capacity in the cell.
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论文发表日期：2020-03-30
页数：5( 36-40 )
英文信息