A comparative in vitro study on the cerumenolytic effect of docusate sodium versus 2.5％sodium bicarbonate using UV-visible absorption spectroscopy
Chompunut Srisukhumchai1
Pornthep Kasemsiri2
Teeraporn Rattanaanekchai2
Somchai Srirompotong2
Umaporn Yordpratum3
Chadamas Sakolsinsiri4
1.Department of Otorhinolaryngology, Srinagarind Hospital, Faculty of Medicine at the Khon Kaen University, Khon Kaen, Thailand2.Department of Otorhinolaryngology, Srinagarind Hospital, Faculty of Medicine at the Khon Kaen University, Khon Kaen, Thailand;Khon Kaen Ear Hearing and Balance Research Group, Khon Kaen University, Thailand3.Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand4.Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand
摘要：Objective: To compare cerumenolytic effects of docusate sodium and of 2.5％ sodium bicarbonate -In vitro study;observe characteristics of the solution, using ultraviolet-visible (UV/Vis) spectroscopy, and measurement of cholesterol levels.Methods: Samples of human cerumen were mixed to form a relatively homogenous paste. Samples of about 500 mg were weighed and packed at the bottom of the test tubes. To each tube was added 1.5 ml of either docusate sodium or 2.5％sodium bicarbonate. Tubes were incubated at 36.4 °C in a water bath for 15, 30 or 60 min. Following incubation, the supernatant solution was pipetted into a cuvette. The cer-umenolytic efficacy was defined as the absorbance (recorded at 350 nm and 400 nm) of the solutions. Results were the average of three replicates. A cholesterol level of each sample was then determined to confirm the result. Results: Turbidity was much greater in tubes containing 2.5％sodium bicarbonate, indicating dissolution of cerumen. Mean difference of absorbance values measured at 350 nm and 400 nm after 15, 30, 60 min digestions were 1.93 [95％CI 1.49-2.38, p-value <0.001] and 1.81 [95％CI 1.21-2.41, p-value <0.001], respectively. Furthermore, levels of cholesterol were greater in tubes containing 2.5％sodium bicarbonate solution after digestion than in tubes containing docusate sodium; 11 mg/dl [95％CI 1.47-24.14, p-value=0.083] Conclusion: Both spectrophotometric and cholesterol level assessments suggest that 2.5％ sodium bi-carbonate has a higher cerumenolytic effect than docusate sodium. In other words, cerumen can be dissolved in 2.5％sodium bicarbonate much better than docusate sodium in a time-dependent manner.
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论文发表日期：2020-09-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 99-102 )
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