MECOM promotes supporting cell proliferation and differentiation in cochlea
Peipei Chen1
Na Zuo2
Cheng Wu2
Jun Ma2
Yao Li2
Junfei Gu3
Wen Li4
Shaofeng Liu2
1.Department of Histology and Embryology,Wannan Medical College,Wuhu,China2.Department of Otolaryngology-Head and Neck Surgery of Yijishan Hospital,Wannan Medical College,Wuhu,China3.Hospital of Wannan Medical College,Wuhu,China4.Eye&ENT Hospital of Fudan University,Shanghai,China
摘要：Permanent damage to hair cells (HCs) is the leading cause of sensory deafness. Supporting cells (SCs) are essential in the restoration of hearing in mammals because they can proliferate and differentiate to HCs. MDS1 and EVI1 complex locus (MECOM) is vital in early development and cell differentiation and reg-ulates the TGF-β signaling pathway to adapt to pathophysiological events, such as hematopoietic pro-liferation, differentiation and cells death. In addition, MECOM plays an essential role in neurogenesis and craniofacial development. However, the role of MECOM in the development of cochlea and its way to regulate related signaling are not fully understood. To address this problem, this study examined the expression of MECOM during the development of cochlea and observed a significant increase of MECOM at the key point of auditory epithelial morphogenesis, indicating that MECOM may have a vital function in the formation of cochlea and regeneration of HCs. Meanwhile, we tried to explore the possible effect and potential mechanism of MECOM in SC proliferation and HC regeneration. Findings from this study indicate that overexpression of MECOM markedly increases the proliferation of SCs in the inner ear, and the expression of Smad3 and Cdkn2b related to TGF signaling is significantly down-regulated, corre-sponding to the overexpression of MECOM. Collectively, these data may provide an explanation of the vital function of MECOM in SC proliferation and trans-differentiation into HCs, as well as its regulation. The interaction between MECOM, Wnt, Notch and the TGF-β signaling may provide a feasible approach to induce the regeneration of HCs.
机标关键词：cellcochleadifferentiationmecomproliferationpromotessupporting
论文发表日期：2022-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 59-66 )
英文信息