Preparation and application of amniotic membrane matrix

Zhang Yu-min; Zhang Nai-li; Zhou Mo; Ma Shao-ying; Wang Xu-sheng; Xu Wei-jun; Li Bao-xing

doi:10.3969/j.issn.2095-4344.2013.34.021

Abstract： BACKGROUND:Amniotic membrane, an immune privilege tissue graft, containing a variety of cytokines, has biological characteristics of anti-inflammation, anti-adhesion, alleviating pain and promoting epithelization, which has been applied in clinic widely. <br> OBJECTIVE:To review the progress of preparation of amniotic membrane matrix. <br> METHODS:An online search of CNKI (before 2012) and Medline databases (from 1999 to 2012) was performed for articles using keywords of“amniotic membrane matrix, processing, sterilization”in Chinese and English, <br> respectively. Relevant articles were summarized from four aspects of amniotic membrane biological characteristics, immunology characteristics, preparation methods and sterilization methods. A total of 98 articles were included. According to inclusion and exclusion criteria, a number of 28 articles were retained at last. <br> RESULTS AND CONCLUSION:Amniotic membrane, as a natural biomaterial consisting of collagen, glycoprotein, proteoglycan, integrin, laminin, and expressing a variety of cytokines and proteins related to mRNA, provides abundant nutrients for cell growth and differentiation. Amniotic membrane is generally thought as allow immunogenicity graft without HLA-Ⅱ antigen. However, there are some reports that amniotic membrane contains a small amount of HLA-Ⅱ antigen and have certain immunogenicity. According to the preparation ways, amniotic membranes are classified into fresh amniotic membrane, freeze drying amniotic membrane, acel ular amniotic membrane and, they have their respective advantages and disadvantages. It is one of the hot spots to further enhance its allaspects of performance by modification.