The inhibitor effect of dihydroartemisinin on liver fibrosis of mice induced by CCl4

GUO Yongze; SHAN Tieying; REN Haixia; CAI Li; LI Xiaotian; WANG Jianhua; LIU Xiaodong; CUI Xingliang

doi:10.3969/j.issn.1671-6450.2016.05.021

Abstract：Objective To investigate the inhibitory effect of ( DHA) on hepatic fibrosis induced by CCl4 in mice and its mechanism.Methods From October 2013 to May 2014, in the Central Laboratory of Hebei University of Engineering Medical College, 50 mice were divided into 3 groups:control group ( n =10) , model group ( n =20) and treatment group ( n =20).Model group and treatment group were treated with intraperitoneal injection of carbon tetrachloride (CCl4) to pre-pare the model of liver fibrosis mice.Mice in the treatment group were administered with 20 μg/g, 1 times a day, 12 times, the control group and the model group were given DMSO normal saline for 1 times a day, 12 times.HE and Masson's trichrome staining were used to observe the pathological changes of liver tissue; colorimetric method were used for the determination of the content of hydroxyproline in liver tissue; Western blot method were used for the detection of matrix metalloproteinase 13 (MMP-13) tissues and the expression level of inhibit factor 1 (TIMP-1).Results The HE and Masson staining results con-firmed the successful establishment of animal model of liver fibrosis in mice.In model group mice’ s liver, fatty degeneration of liver cell were found, marked hyperplasia of fibrous tissue, reduce the liver tissue in MMP-13 expression, and hydroxypro-line content and TIMP-1 expression increased, and MMP-13/TIMP-1 value were decreased than the control group ( P <0.05).Treatment group after DHA therapy, mice liver fibrous tissue hyperplasia decreased, increased liver tissue’s MMP-13 expression, and hydroxyproline content and TIMP-1 expression decreased than the model group ( P <0.05).Conclusion Dihydroartemisinin has the function of anti-hepatic fibrosis, which may be as a new type of anti-hepatic fibrosis drug.