Tissue expression of protein and mRNA of MMP-13 in murine allergic contact dermatitis
XU Lei
TIAN Jing
CAI Limin
BAI Bingxue
Abstract：Objective To detect the role of MMP-13 in murine chronic allergic contact dermatitis and determine the relationship and significance between skin inflammation and expression of MMP-13.Methods The experiment was carried out in central laboratory of the first affiliated hospital of Harbin Medical University from January 2016 to September 2016.One hundred and forty of mice were randomly divided into as follows:blank control group,model group,CL-82198 group(group1=CL-82198 0.5 mmol/L,group2=CL-82198 1.25 mmol/L).Chronic ACD model was established, skin inflammatory re-sponse was observed.The expression of mRNA and protein level of MMP-13 in the skin was detected using RT-PCR and im-munohistochemical staining methods.Results (1)The expression of mRNA level and protein level of MMP-13 was signifi-cantly up regulated in model group as compared with that of the control group(t=3.30,χ2=18.65,all P<0.001).(2) The Skin inflammation performance and pathohistological changes in CL-82198 group were significantly decreased than that of model group.The levels of mRNA and protein of MMP-13 in CL-82198 group were significantly decreased than the model group(t=2.16,t=2.95,all P<0.001,χ2=4.77,P=0.029,χ2=16.57,P<0.001).Compared with CL-82198 group 1,the levels of mRNA and protein of MMP-13 were increased significantly in CL-82198 group 2(t=0.79,P<0.001,χ2=4.06,P=0.044).Conclusion (1)The MMP-13 was highly expression in murine chronic ACD,suggesting that MMP-13 participate the development of murine chronic ACD.(2)The MMP-13 inhibitor CL-82198 inhibited DNCB induced skin in-flammation and thus may play role in ACD treatment.
Keywords：MMP-13Allergic contact dermatitisCL-82198Murine
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1269-1272,1276 )