Comparative Study on the Anti Atherosclerosis Function of Huoxue Recipe and Yang He Decoction in apoE-/-Mice Combined with Nonalcoholic Fatty Liver Disease

JIANG Nan; YUAN Xiao-wen; XUE Xin; TAO Xu-guang; QIAO Yan-xue; LI Rui; CHEN Bin; LI Yu-mei; MA Ya-luan

Abstract：Objective:To observe and compare the effect of Huoxue-Fang and Yanghe-Decoction on AS and NAFLD of apoE-deficient mice with Western diet (WD) by modulating systemic immune responses,liver and aortic immune responses.Methods:In the present study,10 week-old homozygous female apoE-/-mice were fed with a normal chow diet,a high cholesterol WD and WD supplemented with Huoxue-Fang and Yanghe-Decoction.After 18 weeks,the proportions of monocyte and subtypes of monocyte,and their surface receptor TLR4 in peripheral blood were detected by flow cytometry.Serum cytokines were detected by suspension array technology.The pathogenesis of aorta was detected by HE staining,the pathogenesis liver was detected by Oil-Red O staining and cytokines in aorta and liver were detected by real-time PCR.Results:18-week WD increased the levels of TC and LDL in apoE-/-mice.Hyperlipidemia increased the proportions of monocyte and Ly6C + +,and expression of their surface receptor TLR4 in apoE-/-mice;and induced serum IL-10,IL-1b,MCP-1,M-CSF and TNF-a levels.In aorta,18-week WD induced atherosclerosis lesions of aorta in apoE-/-mice,increased expressions of cytokines TNF-a,MCP-1,IFN-γ,NOS2 and IL-1b expression.In liver,18-week WD induced NAFLD in apoE-/ mice,increased expressions of cytokines TNF-a,IL-6,MCP-1,IFN-γ,NOS2 and IL-1b expression.After 18 weeks admistration of Houxue-Fang,Yanghe-Decoction on ApoE-/-mice with WD,these medicines did not change the serum lipid levels significantly.Houxue-Fang decreased the proportions of monocyte and Ly6C + +.Huoxue-Fang and Yanghe-Decoction decreased TLR4expression in monocyte and Ly6C + + Huoxue-Fang lowered serum IL-10,IL-1b,MCP-1,TNF-a and M-CSF level,and Yanghe-Decoction just decreased TNF-a level.In liver,Huoxue-Fang and Yanghe-Decoction decreased steatosis.Huoxue-Fang and Yanghe-Decoction decreased expressions of cytokines TNF-a,MCP-1,IFN-γ,NOS2 and IL-1b in aorta and liver.And Huoxue-Fang showed more effect than Yanghe-Decoction on inhibiting steatosis and inflammatory cytokines in aorta and liver.Conclusion:Hyperlipidemia induced systemic immune responses,liver and aortic inflammatory disproportions in apoE-/-mice.Huoxue-Fang and Yanghe-Decoction can modulate these inflammatory disproportions by different degrees and in a lipids-lower-independent manner to inhibit or delay the development of AS and NAFLD.