Effect of Zuogui Jiangtang Jieyu Recipe on the Expression of Glucocorticoid Receptor in HPA Axis and Hippocampus of Diabetic rats with Depression
HAN Yuan-shan
ZHAO Hong-qing
YANG Hui
DU Qing
MENG Pan
LIU Zhuo
WANG Yu-hong
Abstract：Objective:To explore the regulation of HPA axis and glucocorticoid receptor in the compound Chinese medicine Zuogui Jiangtang Jieyu Prescription in Diabetic mellitus with depression and to clarify its possible mechanism of protection in hippocampus.Methods:Intravenous injection of streptozotocin combined with chronic stress continuously to replicate the diabetic rats model with depression,and divided into the model group,low,middle,and high dose of prescription groups,positive drug group.Normal rats were used as control group simultaneously.After the last administration,rats in each group were carried out the detection of blood glucose level and forced swimming test,using HE staining detect hippocampal damage.Elisa kit was used to disclose plasma CRH,ACTH and CORT levels,and Westernblotting to detect the expression of hippocampal glucocorticoid receptor.Results:After giving Zuogui Jiangtang Jieyu Prescription interventions,blood glucose significantly decreased in model rats,and its depression-like behavior alleviated,hippocampal damage be mitigated,ACTH,CORT and GR.levels be reversed.Conclusion:Zuogui Jiangtang Jieyu Prescription could obviously improve the blood glucose and depression-like behaviors in diabetic rats with depression,and protected hippocampal neurons,which role might be associated with up-regulated expression of GR.
Keywords：GlucocorticoidGlucocorticoid receptordiabetes mellitus with depressionZuogui Jiangtang Jieyu Prescription
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1710-1713 )