DOI: 10.1002/ame2.12511
Antiviral effects and mechanism of Qi pi pill against influenza viruses
Chengcheng Zhang1
Jing Gao2
Meiyue Dong3
Carolina Q.Sacramento4
Ping Li5
Xiangyu Lian6
Lingyuan Fan6
Lijun Rong4
Ruikun Du6
Jingzhen Tian1
Qinghua Cui6
1.College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China2.The Third People's Hospital of Qingdao,Qingdao,Shandong,China3.Innovation Research Institute of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan,Shandong,China4.Department of Microbiology and Immunology,College of Medicine,University of Illinois at Chicago,Chicago,Illinois,USA5.Department of Medicinal Chemistry,Key Laboratory of Chemical Biology(Ministry of Education),School of Pharmaceutical Sciences,Shandong University,Jinan,Shandong,China6.Qingdao Academy of Chinese Medicinal Sciences,Shandong University of Traditional Chinese Medicine,Qingdao,Shandong,China
摘要：Background:Qi pi pill(QPP),which contains Renshen,Baizhu,Fuling,Gancao,Chenpi,Shanyao,Lianzi,Shanzha,Liushenqu,Maiya,and Zexie,was recommended for pre-venting and treating COVID-19 in Shandong Province(China).However,the mech-anism by which QPP treats infectious diseases remains unclear.This study aims to investigate the therapeutic effect of QPP in vitro and on acute influenza infection in mice,exploring its mechanism of action against influenza A virus(IAV).
Methods:The in vitro activity of QPP was assessed using dose-response curve analy-sis and titer reduction assay,and its antiviral mechanism was identified in vitro by real-time polymerase chain reaction(PCR),time-of-addition,and enzymatic assays.The antiviral efficacy of QPP was further evaluated in vivo using BALB/c mice infected with IAV.At the same time,each single Chinese herbal medicine in QPP was evaluated to preliminarily identify those with antiviral effects.
Results:In vitro results showed that QPP exhibited a higher potency antiviral effect against both influenza A and B viruses,inhibiting viral RNA replication and release by targeting RNA-dependent RNA polymerase and neuraminidase.Additionally,QPP sig-nificantly decreased the expression of inflammatory cytokines in A549 cells.In vivo study revealed that QPP significantly reduced the lung index and viral load in lung tissue of mice infected with IAV.Renshen,Gancao,Zexie,and Lianzi were the Chinese herbal medicines from QPP that showed anti-IAV activity.
Conclusion:The antiviral activity of QPP targets IAV replication and release,cy-tokine modulation in host cells,and provides protection in mice with acute influenza infection.
机标关键词：mechanismagainstantiviraleffectsinfluenzapillviruses
论文发表日期：2025-08-30
在线出版日期：2025-11-05（本平台首次上网日期，不代表文献的发表时间）
页数：12( 1364-1375 )
英文信息