DOI: 10.1002/ame2.70046
SOX6 enhances vascular smooth muscle cell phenotypic switching and elevates blood pressure by activating autophagy
Qianhui Ling1
Xilan Dong2
Liyan Mao1
Chengjun Huang1
Linjing Cong1
Haizeng Zhang1
Jun Cai1
Zhenzhen Chen1
1.Beijing Institute of Heart Lung and Blood Vessel Diseases,Beijing Anzhen Hospital of Capital Medical University,Beijing,China2.State Key Laboratory of Cardiovascular Disease,Hypertension Center,Fuwai Hospital,National Center for Cardiovascular Diseases,Peking Union Medical College,Chinese Academy of Medical Sciences,Beijing,China
摘要：Background:SOX6 has been shown to play a crucial role in the development of the cardiovascular system.However,its potential role in hypertension and vascular function remains unclear.
Methods:In vascular smooth muscle cells(VSMCs),we employed gain-and loss-of-function approaches combined with RNA sequencing,autophagy flux assessment,and phenotype characterization.Additionally,we established a mouse model with Sox6 overexpression via adeno-associated virus 2(AAV2)to validate the findings in vivo.
Results:We validated the increased expression of SOX6 in hypertension both in vitro and in vivo.Genetic silencing of Sox6 in VSMCs attenuated the phenotypic switching induced by angiotensin Ⅱ.Conversely,in vivo overexpression of Sox6 led to a significant elevation in blood pressure and promoted vascular remodeling.Mechanistically,SOX6 was shown to regulate phenotypic switching via an autophagy-dependent pathway.Specifically,Sox6 overexpression augmented VSMC autophagy and facilitated phenotypic switching,whereas Sox6 knockdown yielded opposite outcomes.Modulation of autophagy using 3-MA or RAPA could effectively counteract the effect mediated by SOX6.
Conclusions:Our findings revealed that SOX6 regulates VSMC plasticity and elevates blood pressure by activating autophagy.Therefore,SOX6 inhibition potentially represents a novel strategy for treating hypertension and vascular remodeling.
机标关键词：pressurebloodcellactivatingautophagyelevatesenhancesmuscle
论文发表日期：2025-08-30
在线出版日期：2025-11-05（本平台首次上网日期，不代表文献的发表时间）
页数：16( 1400-1415 )
英文信息