DOI: 10.1002/ame2.70027
Rapid severe hypertension and organ damage in two-kidney two-clip rats produced by different sizes of clips
Jia-Sheng Tian1
Qi-Sheng Ling1
Yu-Chen Wei2
Dao-Xin Wang1
Chao-Yu Miao3
1.Department of Pharmacology,Second Military Medical University/Naval Medical University,Shanghai,China2.School of Medicine,Shanghai University,Shanghai,China3.Department of Pharmacology,Second Military Medical University/Naval Medical University,Shanghai,China;School of Medicine,Shanghai University,Shanghai,China
摘要：Background:The two-kidney two-clip(2K2C)rat model is widely used in hyperten-sion,stroke,and drug studies.However,knowledge of this model is quite limited.
Methods:In this study,U-shaped silver clips with inner diameters of 0.3,0.25,and 0.2 mm were used to narrow bilateral renal arteries,inducing different extents of blood flow reduction for preparing 2K2C rats.Blood pressure(BP)was continuously measured during 2K2C procedures until 10h after 2K2C,and long-term assessments of BP,organ damage,and serum biochemistry were performed at 1,2,4,and 6 weeks.
Results:BP increased immediately when the 2K2C rats regained consciousness from anesthesia,with a 100％incidence of hypertension on the day of operation,and in-creased to a plateau at 2 weeks for 0.2 mm clips(248 mmHg),4 weeks for 0.25 mm clips(219 mmHg),and perhaps 6 weeks for 0.3 mm clips(206 mmHg).BP levels displayed a negative correlation with clip sizes.Organ damage,including aortic and cardiac hyper-trophy,cerebrovascular and cardiovascular injury,and renal atrophy or compensatory enlargement,occurred as early as 1 or 2 weeks after 2K2C.Routine serum biochemistry indicated organ dysfunction primarily at 4-6 weeks in 0.2 mm clips,whereas aspartate aminotransferase appeared a sensitive biomarker for early organ damage.
Conclusion:2K2C can produce severe hypertension and multi-organ damage in a short time.2K2C rats in 0.2 mm clips can be used as a hypertensive emergency model.This study provides crucial insights for guiding the development of targeted interventions.
机标关键词：hypertensiondifferentclipsrapiddamageratsorganproduced
论文发表日期：2025-08-30
在线出版日期：2025-11-05（本平台首次上网日期，不代表文献的发表时间）
页数：12( 1456-1467 )
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