DOI: 10.1002/ame2.70049
Establishment and evaluation of a novel rat model of the fourth ventricle hemorrhage
Chuhua Fu1
Aoqi Liu2
Yijia He1
Fei Li3
Zhouyang Jiang3
Peiwen Guo3
Zhi Chen3
Yujie Chen3
Hua Feng3
1.Department of Neurosurgery and State Key Laboratory of Trauma and Chemical Poisoning,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China;Department of Neurosurgery,Jingmen People's Hospital,Jingchu University of Technology Affiliated Renmin Hospital,Jingmen,China;Chongqing Clinical Research Center for Neurosurgery,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China;Chongqing Key Laboratory of Intelligent Diagnosis,Treatment and Rehabilitation of Central Nervous System Injuries,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China2.Department of Neurosurgery,Jingmen People's Hospital,Jingchu University of Technology Affiliated Renmin Hospital,Jingmen,China3.Department of Neurosurgery and State Key Laboratory of Trauma and Chemical Poisoning,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China;Chongqing Clinical Research Center for Neurosurgery,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China;Chongqing Key Laboratory of Intelligent Diagnosis,Treatment and Rehabilitation of Central Nervous System Injuries,Southwest Hospital,Third Military Medical University(Army Medical University),Chongqing,China
摘要：Background:Hemorrhagic expansion into the fourth ventricle is an independent risk factor for poor outcomes in intraventricular hemorrhage(IVH)patients.However,to date,available animal models of IVH are limited to models of supratentorial ven-tricular hemorrhage,and there are no specific models of fourth ventricle hemor-rhage.This limitation hinders comprehensive basic research and the understanding of the pathophysiological changes that occur following fourth ventricle hemorrhage.Therefore,the development of an animal model of fourth ventricle hemorrhage is highly important.
Methods:In this study,a novel rat model of fourth ventricle hemorrhage was estab-lished via autologous blood injection through the foramen of Magendie.Anesthetized rats were positioned in a stereotaxic apparatus with their heads tilted downward at an angle of approximately 20° relative to the vertical axis.A needle was inserted through the foramen,and autologous blood obtained from the rat's heart was injected into the fourth ventricle via a microinfusion pump.Systematic evaluations of the model were conducted using small-animal magnetic resonance imaging,histopathological analysis,and neurological function assessment.
Results:The rats developed stable and reproducible fourth ventricle hematomas and ventricular dilation.They also exhibited acute-phase hydrocephalus and pathological features of perilesional brain tissue injury,with observed neurological deficits compa-rable to patients with fourth ventricle hemorrhage.
Conclusion:This model successfully recapitulates the clinicopathological and patho-physiological characteristics of patients with fourth ventricle hemorrhage and can be utilized for further investigation into the pathophysiological mechanisms underlying posthemorrhagic hydrocephalus and perilesional brainstem tissue injury.
机标关键词：evaluationnovelmodelestablishmentfourthhemorrhageventricle
论文发表日期：2025-08-30
在线出版日期：2025-11-05（本平台首次上网日期，不代表文献的发表时间）
页数：13( 1480-1492 )
英文信息