DOI: 10.1002/ame2.70084
Comprehensive study of Algerian nettles:Phenolic content and multifunctional activities of Urtica pilulifera and Urtica urens
Hasna Bouhenni1
Koula Doukani2
Bojana Danilović3
Jelena Zvezdanović3
Dragan Cvetković3
Fahadul Islam4
Sherouk Hussein Sweilam5
Gehan Ahmed Othman6
Talha Bin Emran4
1.Laboratory of Agrobiotechnology and Nutrition in Semi-Arid Zones,Faculty of Nature and Life Sciences,Ibn Khaldoun University,Tiaret,Algeria2.Laboratory of Agrobiotechnology and Nutrition in Semi-Arid Zones,Faculty of Nature and Life Sciences,Ibn Khaldoun University,Tiaret,Algeria;Laboratory of Sciences and Techniques of Animal Production,Faculty of Nature and Life Sciences,Abdelhamid Ibn Badis University,Mostaganem,Algeria3.University of Niš,Faculty of Technology,Leskovac,Serbia4.Department of Pharmacy,Faculty of Health and Life Sciences,Daffodil International University,Dhaka,Bangladesh5.Department of Pharmacognosy,College of Pharmacy,Prince Sattam Bin Abdulaziz University,Al-Kharj,Saudi Arabia;Department of Pharmacognosy,Faculty of Pharmacy,Egyptian Russian University,Cairo,Egypt6.Department of Biology,King Khalid University,Abha,Saudi Arabia
摘要：Background:This study investigated the phenolic profile,antioxidant capacity,anti-bacterial effect,and antihemolytic activity of nettle leaves from two understudied Algerian species,Urtica pilulifera and Urtica urens.
Methods:Urtica pilulifera and Urtica urens leaves extracts were prepared by maceration using methanol and distilled water respectively.Their phytochemical analysis(total phe-nolic content,flavonoids,hydrolysable and condensed tannins)was determined.The chemical profle of these extracts was performed using ultra-high-performance liquid chromatography diode array detection tandem mass spectrometry.Antioxidant activity(using the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging method),antibacterial activity(using disk diffusion method),and antihemolytic activity were carried out.
Results:Results reveal high levels of flavonoids,tannins,alkaloids,and terpenoids.Several classes of compounds were identified in the extracts.Phenolic acid and other acids and their derivatives and flavonoids were detected in the extracts,with 4-O-caffeoyl-quinic acid and 5-O-caffeoyl-quinic acid being the main constituents in both extracts.Caffeic acid was also the main constituent present only in U.urens extract.For antioxidant activity of the metha-nolic extract of U.pilulifera and the aqueous extract of U.urens,the percentage inhibition value(82.76％and 59.06％)and the half-maximum inhibitory concentration(IC50)value(302 and 423μg/mL),respectively,were obtained.Antibacterial activity of nettle extracts(10mg/mL)demonstrated the sensitivity of pathogenic strain susceptibility.The strongest antibacterial effect on tested strains was found in the aqueous extract of U.urens against Staphylococcus aureus(21±0.41mm)compared to the methanolic extract of U.pilulifera(16±0.40mm).The antihemolytic activity in the methanolic and aqueous extracts was 76.26％and 60.67％,re-spectively.The methanolic extract exhibited exceptional antihemolytic effect,with an IC50 value of 327μg/mL,whereas the aqueous extract had an IC50 value of 412μg/mL.
Conclusions:The study confirms the presence of bioactive substances in the nettle species,including flavonoids and tannins,which possess antioxidant,antibacterial,and antihemolytic properties,and can be processed into food and pharmaceutical products.
机标关键词：contentstudyactivitiesalgeriancomprehensivemultifunctionalnettlesphenolic
论文发表日期：2025-10-30
在线出版日期：2025-12-16（本平台首次上网日期，不代表文献的发表时间）
页数：19( 1794-1812 )
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