DOI: 10.1002/ame2.70091
Refining the Sox10Dom/+mouse model:A new breeding strategy with relevance to Hirschsprung disease genetics
Chaoting Lan1
Jiazhang Chen2
Shenwei Huang2
Yide Mu2
Qiuhua Wang3
Xin Zhong3
Ning Tang3
Xinying Zhao4
Jieting Lu4
Yuxin Wu4
Lihua Huang4
Jixiao Zeng4
Wei Zhong5
Yu Ouyang3
Qiuming He1
Yan Zhang4
1.Guangzhou Women and Children's Medical Center Liuzhou Hospital,Liuzhou,China;Department of Pediatric Surgery,Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou,China2.Department of Pediatric Surgery,Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou,China;Guangzhou Medical University,Guangzhou,China3.Guangzhou Women and Children's Medical Center Liuzhou Hospital,Liuzhou,China4.Department of Pediatric Surgery,Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou,China5.Department of Pediatric Surgery,Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease,Guangzhou Women and Children's Medical Center,Guangzhou Medical University,Guangdong Provincial Clinical Research Center for Child Health,Guangzhou,China;Department of Neonatal Surgery,Guangdong Women and Children Hospital,Guangzhou,China
摘要：Background:The traditional Sox10Dom/+mouse breeding strategy is costly and time-consuming,so this study aims to optimize the breeding method and improve the sci-entific research efficiency.
Methods:We select the offspring from mating B6C3Fe Sox10Dom/+male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.
Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.
Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phe-notypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10Dom/+mice.
机标关键词：breedingstrategymousemodelwithdiseasegeneticshirschsprung
论文发表日期：2025-10-30
在线出版日期：2025-12-16（本平台首次上网日期，不代表文献的发表时间）
页数：10( 1866-1875 )
英文信息