DOI: 10.1002/ame2.70097
An in vivo electrophysiological preparation for mechanical,electrical and optical stimulation of sensory neurons that innervate murine bone
Michael Morgan1
Hoi Ying Lee1
Aung Aung Kywe Moe2
Jenny Thai1
Jackson Hart1
Jason J.Ivanusic1
1.Department of Anatomy and Physiology,University of Melbourne,Melbourne,Victoria,Australia2.Department of Anatomy and Physiology,University of Melbourne,Melbourne,Victoria,Australia;Department of Medical Imaging and Radiation Sciences,School of Primary and Allied Health Care,Monash University,Melbourne,Victoria,Australia
摘要：In this study,we aimed to develop an in vivo electrophysiological bone-nerve prepa-ration to record the activity of peripheral sensory neurons that innervate the mu-rine tibia.A small nerve that innervates the tibial marrow cavity was identified in isoflurane-anesthetized C57BL/6 mice,and placed over a platinum hook electrode for extracellular recording.Whole-nerve activity was amplified,filtered and sampled at 20kHz using PowerLab(ADInstruments).A cannula was placed into the marrow cavity to deliver mechanical stimuli(by pressurizing with injection of saline)and/or capsaicin.Optical stimulation was achieved by application of 473 nm blue light(1 Hz,0.25-0.5 ms,0-12.5 mW/mm)to the tibial marrow cavity in Wnt1-Cre;IoxP-ChR2 mice.Murine bone afferent neurons responded to high threshold noxious mechanical stimulation,coded for the intensity of mechanical stimulation,could be sensitized by capsaicin,and did not suffer stimulus-evoked fatigue at 10-minute interstimulus inter-vals.Electrical and optical stimulation within the marrow cavity evoked action poten-tials with conduction velocities in the Aδ and/or C fiber range.These new approaches to recording the activity of bone afferent neurons will allow us to take advantage of transgenic and optogenetic tools to further our understanding of mechanisms that generate and maintain bone pain in the future.
机标关键词：thatvivoboneelectricalinnervatemechanicalmurineneurons
论文发表日期：2025-10-30
在线出版日期：2025-12-16（本平台首次上网日期，不代表文献的发表时间）
页数：6( 1915-1920 )
英文信息