DOI: 10.1002/ame2.70098
Evaluation of a modified triple-combination anesthesia using dexmedetomidine in mice
Masaki Watanabe1
Ryosuke Nakanishi1
Tomoki Omori1
Takeru Sasaki1
Atsushi Asano2
Nobuya Sasaki1
1.Laboratory of Laboratory Animal Science,Joint Facu2.Laboratory of Laboratory Animal Science and Medici
摘要：Background:Ketamine is a widely used anesthetic in animal research,but its use is strictly regulated in several countries,including Japan and China.As an alternative,the medetomidine-midazolam-butorphanol(MMB)combination is commonly used in Japan.However,medetomidine is a racemic mixture containing the inactive R-enantiomer,which may reduce anesthetic predictability and safety.
Objective:The aim of the study was to evaluate the efficacy and safety of a modified anesthetic combination(dMMB),in which dexmedetomidine replaces medetomidine,across three commonly used mouse strains(ICR,C57BL/6,BALB/c).
Methods:Male and female mice were administered either MMB or dMMB sub-cutaneously.Anesthetic depth,recovery profiles,heart rate,SpO2,body temper-ature,ocular opacity,and blood glucose levels were assessed.Atipamezole was used to reverse anesthesia,and thermoregulatory recovery was monitored postinjection.
Results:dMMB produced similar anesthetic depth to MMB,with faster and more con-sistent recovery,particularly in males.Body temperature recovery was significantly enhanced in dMMB-treated B6 males.No significant differences in side effects(ocu-lar opacity or blood glucose levels)were observed between protocols,though strain-specific glucose elevations were noted in dMMB-treated males.
Conclusion:dMMB is a safe,effective,and ketamine-free injectable anesthetic pro-tocol,offering advantages in recovery and thermoregulation.It may be a valuable alternative in research settings where ketamine is restricted and medetomidine may become unavailable.
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论文发表日期：2025-11-30
在线出版日期：2026-01-09（本平台首次上网日期，不代表文献的发表时间）
页数：14( 2099-2112 )
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