DOI: 10.1002/ame2.70040
Identification of ferroptosis-related genes involved in chronic obstructive pulmonary disease based on bioinformatics analysis
Xuejing Luan1
Jinghui Xie1
Lu Zhang2
Xiao Ma2
Lichangtian Jiao3
Jie Zhu4
1.College of Integrated Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei,China2.The First Clinical Medical College,Anhui University of Chinese Medicine,Hefei,China3.College of Acupuncture and Tuina,Anhui University of Chinese Medicine,Hefei,China4.College of Integrated Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei,China;The First Clinical Medical College,Anhui University of Chinese Medicine,Hefei,China
摘要：Background:Chronic obstructive pulmonary disease(COPD)is a type of chronic res-piratory disease.Studies confirmed that ferroptosis was involved in the progression of COPD,and its related mechanism is not clear.The aim of this study was to identify ferroptosis-related genes and reveal its pathological application in COPD.
Methods:First,we downloaded two datasets from the Gene Expression Omnibus(GEO)to obtain the differential genes of COPD.Ferroptosis-related genes were ob-tained from the ferroptosis database,FerrDb.Next,we obtained the key genes in COPD rat to identify potential biomarkers using quantitative real-time polymerase chain reaction.Ferroptosis and inflammation were assessed using hematoxylin and eosin staining,lung function tests,and transmission electron microscopy(TEM).
Results:These results were used to construct a COPD risk model with six key genes and explore the immunological characteristics of these genes.The resulting mo-lecular subtype construction confirmed the importance of the key genes in COPD.Furthermore,we proved that ferroptosis occurred in the COPD rat model and identi-fied the six key genes in rat lung tissue.TEM showed significant functional impairment and structural alterations in mitochondria,which is the key site of ferroptosis.
Conclusion:Our COPD risk model,incorporating six key genes,highlighted their im-munological roles in COPD using bioinformatics analysis and in vivo experiments.We hope to provide the basis for the treatment targeting ferroptosis in COPD.
机标关键词：bioinformaticsidentificationferroptosisanalysisgenesbasedchronicdisease
论文发表日期：2025-12-30
在线出版日期：2026-03-25（本平台首次上网日期，不代表文献的发表时间）
页数：21( 2232-2252 )
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