DOI: 10.1002/ame2.70078
Modeling chronic periodontitis in rats:Persistent alveolar bone loss mediated by periodontal pathogens
Maksym Skrypnyk1
Chun Xu2
Shlomo Berkovsky3
Thilini Jayasinghe2
Axel Spahr4
1.Centre for Health Informatics,Australian Institute of Health Innovation,Faculty of Medicine,Health and Human Sciences,Macquarie University,Sydney,New South Wales,Australia;The Charles Perkins Centre,Faculty of Medicine and Health,The University of Sydney,Camperdown,New South Wales,Australia;School of Dentistry,Faculty of Medicine and Health,The University of Sydney,Surry Hills,New South Wales,Australia2.The Charles Perkins Centre,Faculty of Medicine and Health,The University of Sydney,Camperdown,New South Wales,Australia;School of Dentistry,Faculty of Medicine and Health,The University of Sydney,Surry Hills,New South Wales,Australia3.Centre for Health Informatics,Australian Institute of Health Innovation,Faculty of Medicine,Health and Human Sciences,Macquarie University,Sydney,New South Wales,Australia4.School of Dentistry,Faculty of Medicine and Health,The University of Sydney,Surry Hills,New South Wales,Australia
摘要：Background:Although widely used in periodontal research,rodents are naturally re-sistant to periodontitis.Conventional models,such as ligature-induced periodontitis,often fail to sustain defects due to spontaneous tissue regeneration after ligature removal.To address this,we refined a rat ligature-induced model of experimental periodontitis to better mimic the chronic,progressive nature of human periodontitis.
Methods:As a first step,following a split-mouth design,we compared the effective-ness of 3/0 silk thread and 0.008-inch orthodontic wire as ligature materials.Ligatures were applied around the left mandibular first molar for 6,10,and 14days.Periodontal pocket irrigation was performed every second day using a suspension of P.gingivalis,P.intermedia,and S.gordonii.As a second step,we evaluated whether silk-ligature alone,without human periopathogens,would be sufficient to induce a stable and progres-sive periodontal lesion.For that purpose,a silk ligature was removed on day 14,and the bone defect dynamics were monitored at 14-,21-,and 28-days post-removal using micro-CT.
Results:Both wire and silk ligatures,in combination with bacterial irrigation,effec-tively induced rapid interproximal alveolar bone loss.However,silk ligature only,without periodontopathogen colonization,resulted in significantly lower bone loss(1.076±0.22mm vs.2.012±0.374mm;p=0.003)and the induced alveolar bone de-fects gradually resolved again over time.
Conclusions:The proposed rat model of periodontitis is well characterized and repli-cates human disease by sustaining colonization with viable periopathogens,leading to progressive disease with alveolar bone loss.The suggested model is straightforward,easy to establish and can be used reliably in preclinical studies.
机标关键词：modelinglossratsalveolarbonechronicmediatedpathogens
论文发表日期：2025-12-30
在线出版日期：2026-03-25（本平台首次上网日期，不代表文献的发表时间）
页数：13( 2253-2265 )
英文信息