DOI: 10.1002/ame2.70104
Hub genes and diagnostic model associated with mitochondrial function in Alzheimer's disease
Xuchao Zhu1
Ling Zhang2
Chuan Qin3
1.NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Science,CAMS &Comparative Medicine Center,PUMC,Beijing,China;Changping Laboratory,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China2.NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Science,CAMS &Comparative Medicine Center,PUMC,Beijing,China;National Human Diseases Animal Model Resource Center,Beijing,China;National Center of Technology Innovation for Animal Model,Beijing,China;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases,Beijing,China;Key Laboratory of Pathogen Infection Prevention and Control(Ministry of Education),National Institute of Pathogen Biology,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,P.R.China3.NHC Key Laboratory of Human Disease Comparative Medicine,Institute of Laboratory Animal Science,CAMS &Comparative Medicine Center,PUMC,Beijing,China;Changping Laboratory,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,China;National Human Diseases Animal Model Resource Center,Beijing,China;National Center of Technology Innovation for Animal Model,Beijing,China;Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases,Beijing,China;Key Laboratory of Pathogen Infection Prevention and Control(Ministry of Education),National Institute of Pathogen Biology,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing,P.R.China
摘要：Background:Alzheimer's disease(AD)represents the most prevalent neurodegen-erative disorder,with mitochondrial dysfunction being observed in both AD patients and mouse models.Nonetheless,further investigation is required to elucidate the pathogenic genes associated with AD and to develop early diagnostic methodologies centered on mitochondrial function.
Methods:In this study,the dataset GSE132903 was retrieved from the GEO database,encompassing both non-demented(ND)control and AD samples.Through the combi-nation of differential expression gene analysis,weighted gene co-expression network analysis,and intersection with mitochondrial database gene sets,four hub genes as-sociated with AD were identified.These four hub genes were subsequently validated in APP/PS1 and 5xFAD mouse models using molecular biology techniques.
Results:The hub genes identified through bioinformatics analysis include SYNJ2BP,VDAC1,NUBPL,and COX19.Within the GSE132903 dataset,the expression levels of SYNJ2BP,NUBPL,and COX19 were significantly elevated in the AD group compared to the non-demented(ND)group,whereas VDAC1 expression was reduced in the AD group relative to the ND group.Furthermore,in the hippocampus of APP/PS1 and 5xFAD mouse models,the expression patterns of SYNJ2BP and NUBPL were consist-ent with the bioinformatics analysis results.
Conclusion:Hub genes identified here through bioinformatics and molecular biology may help early diagnosis of AD patients and may also help build new AD models to explore its pathogenesis.
机标关键词：alzheimerdiagnosticfunctiongenesmodelwithassociateddisease
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：12( 237-248 )
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