DOI: 10.1002/ame2.70118
Differential roles of EA-TRAPed cells in the anterior cingulate cortex across various intervention times in inflammatory pain
Zi Guo1
Ru Ye1
Lu Guan1
Wei He1
Shuang Qiu2
Xiaomei Shao1
Junfan Fang1
Jianqiao Fang1
Junying Du1
1.Department of Neurobiology and Acupuncture Research,The Third School of Clinical Medicine,Zhejiang Chinese Medical University,Hangzhou,China;Key Laboratory for Research of Acupuncture Treatment and Tranformation of Emotional Diseases,Zhejiang Chinese Medical University,Hangzhou,China2.Department of Neurobiology and Department of Anesthesiology of Second Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,China;Liangzhu Laboratory,Ministry of Education Frontier Science Center for Brain Science and Brain-Machine Integration,State Key Laboratory of Brain-Machine Intelligence,Zhejiang University,Hangzhou,China
摘要：Background:The analgesic effects of multiple electroacupuncture(EA)sessions and single EA sessions differ significantly in pain management.Area 24b(A24b)of the anterior cingulate cortex(ACC)is crucial in pain processing.EA relieves pain by tar-geting and modulating the neuronal activity within this subregion.However,whether the cumulative effect of EA antinociception is connected to A24b mechanisms has remained unclear.
Methods:In our study,we used the Complete Freund's Adjuvant(CFA)model to induce inflammatory pain and the Spared Nerve Injury(SNI)model to induce neuropathic pain,and adult male C57BL/6,FosTRAP,and FosTRAP:Ai9 mice were used as experimental subjects to investigate the cumulative effect of EA antinociception and whether multi-ple EA sessions and a single EA session regulate different neuronal populations in the A24b.
Results:We observed that EA effectively alleviated pain in mice,with three EA ses-sions yielding superior analgesic effects compared to a single session.Using chemi-cal genetics combined with FosCreER technology to activate EA-TRAPed cells in the A24b,we found that pain relief was more pronounced with three EA sessions.Moreover,chemogenetic inhibition of EA-TRAPed cells in the A24b reversed the an-algesic effects of a single EA session but not those of three EA sessions.Fluorescent in situ hybridization results indicated that three EA sessions significantly increased the number of GABAergic neurons in the A24b compared with a single session.Additionally,retrograde tracing revealed that the A24b circuit that monosynaptically innervates EA-TRAPed cells included projections from the central lateral nucleus(CL),lateral mediodorsal thalamic nucleus(MDL),lateral habenula(LHb),dorsal raphe nu-cleus(DR),caudal linear nucleus of the raphe(CLi),dorsal tuberomamillary nucleus(DTM),periventricular hypothalamic nucleus(Pe)and hippocampal fields CA1,CA2,and CA3.These findings suggest that multiple EA sessions and single EA sessions ac-tivated different neuronal populations in the A24b.The enhanced analgesic effect of multiple EA sessions may be attributed to an increase in the proportion of GABAergic neurons within the A24b.
Conclusions:Multiple and single EA sessions recruit distinct neuronal populations in A24b,with the stronger analgesic effect of repeated EA linked to a higher proportion of GABAergic neurons in this region.
机标关键词：cortexvariouscellsacrossanteriorcingulatedifferentialea-traped
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：15( 329-343 )
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