DOI: 10.1002/ame2.70117
Characterization of inflammatory pseudotumors in a large animal model of liver cancer
Erik N.K.Cressman1
Samantha Hicks2
Natalie W.Fowlkes2
Danielle L.Stolley3
Maria Sophia Stenkamp2
1.Department of Interventional Radiology,MD Anderson Cancer Center,The University of Texas,Houston,Texas,USA2.Department of Veterinary Medicine and Surgery,MD Anderson Cancer Center,The University of Texas,Houston,Texas,USA3.Flow Cytometry & Cellular Imaging Core Facility,MD Anderson Cancer Center,The University of Texas,Houston,Texas,USA
摘要：Background:The development of relevant and robust large animal models of hepatocel-lular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this complex problem.One such model,the Oncopig,has been reported to develop tumors of up to 4cm in diameter within 7-14days at sites of in situ vector inoculation.However,the resulting lesions reportedly contained an extensive inflammatory component that has not been evaluated in detail.
Methods:Herein,we describe our results from multiparametric characterization of the lesions generated using liver biopsy cores incubated in vector solution and re-placed in the tissue.The study consisted of 3 animals in 3 cohorts(total of 9 animals)that were evaluated at 14,21,and 28days.CT imaging,immunohistochemistry,multi-plex immunofluorescence,and comprehensive blood analyses were used to quantify composition of the hepatic masses that developed following AdCre inoculation.
Results:The tumors were hypovascular on CT and predominantly composed of CD45+cells with a strong lymphohistiocytic component,with no carcinomas identified.Ki-67 staining showed proliferation of CD45+immune cells but no neoplastic component.To provide further insight,the results are evaluated in the context of tumor growth kinetics.
Conclusion:While progress has been made in generating targetable lesions,achiev-ing a robust large animal model of liver cancer that faithfully recapitulates the human disease remains a challenging goal.
机标关键词：cancerlargemodeltionanimalcharacterinflammatoryliver
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：10( 344-353 )
英文信息