DOI: 10.1002/ame2.70156
Characterizing a murine breast cancer mouse model reveals chromosomal abnormalities in structure and number of single-cell clones and the presence of rare cancer stem cell-like phenotypes
Quy Van-Chanh Le1
Zsuzsa S.Kocsis2
Sisi Liang1
Yanrui Li3
Briony L.Gliddon4
Sheree Bailey5
Anh Thai-Quynh Nguyen3
Tyron Turnbull6
Stuart M.Pitson4
Zsolt Jurányi2
Hien Le7
Ivan Kempson8
1.Future Industries Institute,Adelaide University,Mawson Lakes,South Australia,Australia;School of Pharmacy and Biomedical Science,College of Health,Adelaide University,Adelaide,South Australia,Australia2.Department of Radiobiology and Diagnostic Onco-Cytogenetics,Centre of Radiotherapy,National Institute of Oncology,Budapest,Hungary3.Future Industries Institute,Adelaide University,Mawson Lakes,South Australia,Australia4.Centre for Cancer Biology,Adelaide University and SA Pathology,Adelaide,South Australia,Australia5.School of Pharmacy and Biomedical Science,College of Health,Adelaide University,Adelaide,South Australia,Australia6.School of Physics,Chemistry & Earth Science,College of Science,Adelaide University,Adelaide,South Australia,Australia7.Department of Radiation Oncology,Royal Adelaide Hospital,Adelaide,South Australia,Australia8.Future Industries Institute,Adelaide University,Mawson Lakes,South Australia,Australia;Centre for Pharmaceutical Innovation,School of Pharmacy and Biomedical Science,College of Health,Adelaide University,Adelaide,South Australia,Australia
摘要：Realistic models for cancer research representing disease progression that commen-surately respond to therapeutics consistent with clinical observation are the holy grail for pre-clinical research and screening.Although such an ideal is elusive,well-characterized in vivo models facilitate our understanding of disease,progression,and therapeutic opportunities.Here,we characterize a commonly used syngeneic BALB/c mouse model of triple negative breast cancer(4T1)after establishing tumors in their flanks.Tumors developed at the subcutaneous injection site for all experimental mice and their volumes were monitored.We quantified a rare subset of breast cancer stem-like cells(CSCs),classified as CD44+/CD24-phenotypes in in vitro and ex vivo cell populations.Chromosome numbers in ex vivo metaphase cells were greater than cells cultured in vitro(89.4±3.4,range of 70-132 and 82.6±1.1,range of 70-128;respec-tively).Further,we observed different types of chromosome aberrations,including gap,deletion,exchange,interstitial deletion,terminal deletion,ring,dicentric,and Robertsonian translocations.For both sources of cells,the number of aberrations was dominated by deletions,terminal deletions,and Robertsonian translocations.Ex vivo cells exhibited greater prevalence of deletions and terminal deletions,whereas in vitro cells displayed more ring aberrations and Robertsonian translocations.In conclusion,we successfully characterized cancer cells from a syngeneic mouse model of breast cancer in terms of rare CSC proportion and a variety of chromosomal aberrations,which is useful for understanding tumor traits associated with cancer development and therapeutic action.The data act as a valuable resource for other studies using the 4T1 BALB/c model.
机标关键词：presencestructurecancerstemmousemodelrareabnormalities
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：11( 367-377 )
英文信息