DOI: 10.1002/ame2.70113
Renal vessel-assisted anastomosis improves the abdominal heart transplant model in rats without bilateral lower limb ischemia
Meng Wang1
Wuxia Wang2
Xunfeng Zou3
1.Department of Cardiovascular Surgery,Chest Hospital,Tianjin University,Tianjin,People's Republic of China2.Health Training Center,Medical Service Training Center,The 983rd Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army,Tianjin,People's Republic of China3.Thyroid Breast and Hernia Surgery,Tianjin First Central Hospital,Tianjin,People's Republic of China;Organ Transplant Department,Institute of Transplantation,Nankai University,Tianjin,People's Republic of China
摘要：Background:The traditional method of heterotopic abdominal heart transplantation(HTx)involves cross-clamping the inferior vena cava,which inevitably leads to bilat-eral lower limb ischemia(LI).This study first aimed to investigate the impact of LI on renal function in rats subjected to unilateral nephrectomy(UNx).Second,a modified method utilizing renal vessel-assisted anastomosis in rats with left UNx was com-pared with the traditional method for abdominal HTx.
Methods:Male Sprague-Dawley rats were utilized as subjects for both experimen-tal phases.In experiment 1,the animals were divided into four groups:sham opera-tion group;LI group-rats undergoing occlusion of the abdominal aorta and vena cava below the renal vessels;UNx group-rats with left UNx;and LI+UNx group.All oper-ated animals were monitored for up to 7days for biochemical markers,renal histopa-thology,and survival rates.In experiment 2,we introduced the renal vessel-assisted method as the experimental group and compared it against the traditional method as the control within rat heterotopic HTx models.We assessed operative characteristics,echocardiography results,histological findings,and graft survival.
Results:First,LI resulted in acute kidney dysfunction characterized by a decrease in 7-day survival rates and creatinine clearance rates in both the LI and LI+UNx groups compared to the sham operation and UNx groups.Particularly,histopathological damage in the kidney and liver did not exhibit significant effects during this period.Second,the implementation of the renal vessel-assisted method significantly reduced bleeding volume at suture sites and enhanced the 7-day survival rate compared to the traditional method.
Conclusion:Acute kidney injury was induced by LI postoperation in treated rats.The renal vessel-assisted method demonstrated its effectiveness as a superior alternative that mitigates complications associated with the traditional method.
机标关键词：ischemiaheartmodelratsabdominalanastomosisbilateralimproves
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：9( 407-415 )
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