DOI: 10.1002/ame2.70168
A preclinical rat model for bilateral phrenic nerve stimulation during mechanical ventilation
Jingyi Li1
Mulin Zhang1
Meizhizi Zhang1
Fenqin Xue2
Zhize Gao3
Xiang Qi4
Yongxing Sun5
Zhonghua Shi6
1.Department of Neurosurgery,Intensive Care Unit,Sanbo Brain Hospital,Capital Medical University,Beijing,China;Laboratory for Clinical Medicine,Capital Medical University,Beijing,China2.Laboratory of In Vivo Electrophysiology,Core Facility Center of Capital Medical University,Beijing,China3.Laboratory for Clinical Medicine,Capital Medical University,Beijing,China4.Department of Neurosurgery,Intensive Care Unit,Sanbo Brain Hospital,Capital Medical University,Beijing,China;Laboratory for Clinical Medicine,Capital Medical University,Beijing,China;Department of Anesthesiology,Sanbo Brain Hospital,Capital Medical University,Beijing,China5.Department of Anesthesiology,Sanbo Brain Hospital,Capital Medical University,Beijing,China6.Department of Neurosurgery,Intensive Care Unit,Sanbo Brain Hospital,Capital Medical University,Beijing,China;Laboratory for Clinical Medicine,Capital Medical University,Beijing,China;Department of Physiology,Amsterdam University Medical Centre,Amsterdam,The Netherlands
摘要：Phrenic nerve stimulation(PNS)may preserve diaphragm activation and mitigate multiorgan injury during mechanical ventilation(MV);however,a minimal invasive rat model integrating PNS with MV is lacking.We established an omohyoid muscle-based PNS rat model combined with MV.Bilateral nerves were exposed within 20±2min by transection at the intermediate tendon of omohyoid muscle,minimizing trauma and bleeding.Threshold stimulation(0.6±0.2mA)correlated with body weight.Ventilator-synchronized stimulation increased compound muscle action potentials by～30％,whereas histology confirmed intact nerve.Physiological parameters remained stable throughout ventilation.This model provides a safe and scalable platform for mechanistic and preclinical studies on PNS-mediated protection against MV-induced organ injury.
机标关键词：modelbilateralduringmechanicalnervephrenicpreclinicalstimulation
论文发表日期：2026-02-28
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：6( 416-421 )
英文信息