Role of IL-2-inducible T-cell kinase in anti-HBV type I interferon expression and anti-HBV activity
Mengxin Lin
Jiangfu Liu
Zhijun Su
Yingying Xu
Xiuhua He
Ruyi Guo
Department of Infectious Diseases,First Hospital Affiliated to Fujian Medical University,Quanzhou 362000,Fujian Province,China
摘要：Objective The study aims to investigate the effects of interleukin-2-inducible T-cell kinase(ITK)on hepatitis B virus(HBV)-induced type I interferon(IFN)expression and the antiviral response.
Methods Expression of ITK and type I interferon(IFN)were examined in peripheral blood mononuclear cells from 20 healthy volunteers and 45 HBV-infected patients.Mouse primary hepatocytes were treated with the ITK inhibitor ibrutinib to assess the expression of type I IFN after HBV infection in vitro.Mice were treated with ibru-tinib and evaluated for its effects on HBV infection in vivo.The effects of type I IFN production were explored in cells with an ITK deletion or a double knockout of ITK and suppressor of cytokine signaling 1(SOCS1)engi-neered via CRISPR.
Results The expression levels of ITK and IFN-β were upregulated in patients with acute HBV infection.HBV-in-duced expression of type I IFN was effectively inhibited by treatment with ibrutinib.ITK negatively regulated the expression of SOSC1,whereas inhibiting ITK expression decreased IFN regulatory factor 3 activity and increased SOCS1 expression.In the SOCS1 knockout,the downregulated phenotype of type I IFN expression in ITK-defi-cient cells was reversed after HBV infection.
Conclusion ITK regulated HBV-induced expression of type I IFN by modulating SOCS1.
机标关键词：expressionactivityroleanti-hbvil-2-inducibleinterferonkinaset-cell
论文发表日期：2024-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 38-45 )
英文信息