Reduced serum circRNA_00000367 levels in hepatitis B virus-re-lated hepatocellular carcinoma
Ajuan Zeng1
Jiayi Ma2
Xiaoru Wang2
Rui Sun3
Lei Li1
Huiguo Ding3
Shanshan Wang2
1.Department of Gastroenterology and Hepatology,Beijing Youan Hospital,Capital Medical University,Beijing,China2.Beijing Institute of Hepatology,Beijing Youan Hospital,Capital Medical University,Beijing,China3.Department of Gastroenterology and Hepatology,Beijing Youan Hospital,Capital Medical University,Beijing,China;Beijing Institute of Hepatology,Beijing Youan Hospital,Capital Medical University,Beijing,China
摘要：Objective Circular RNAs(circRNAs)have important roles in the development of hepatitis B virus(HBV)-related cirrhosis and hepatocellular carcinoma(HCC);however,they have not been thoroughly researched.
Methods In this study,we collected blood samples from patients with HCC and cirrhosis for microarray analysis of serum circRNA expression profiles.The expression levels of four circRNAs were further verified in patients with HBV-related HCC and HBV-related cirrhosis using qRT-PCR.TargetScan,miRanda,and circBank were used to predict the interactions between a selected circRNA,circRNA_00000367,and both miRNAs and mRNAs.An interaction network was generated using Cytoscape to elucidate the correlations among the target microRNAs and mRNAs.Gene Ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathways were utilized to interpret the biological functions and signaling pathways of the target genes.
Results A cluster analysis revealed 8 up-regulated and 80 down-regulated circRNAs in HCC group compared with the cirrhosis group.Microarray and qRT-PCR analyses showed that circRNA_0000367 expression was sig-nificantly lower in patients with HBV-related HCC than in patients with HBV-related cirrhosis.Furthermore,the expression levels of circRNA_0000367 were significantly lower in patients with drug-resistant HBV and HBV-re-lated cirrhosis who progressed to HCC.A circRNA_00000367/miRNA/mRNA network analysis showed that the target genes were involved in various biological processes and signaling pathways.
Conclusions CircRNA_0000367 down-regulation in HBV-related cirrhosis may be associated with progression to HCC,providing a potential HBV-related HCC biomarker.
机标关键词：circrnalevelscarcinomahepatitishepatocellularreducedserumvirus-re-lated
论文发表日期：2024-09-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 69-77 )
英文信息