Antibacterial effects of Nanyang Kiwifruit extract toothpaste on common oral pathogens
Tao Liu1
Hongmei Wang2
Hua Li3
Lidan Shi4
Panpan Meng3
Lin Feng3
Rui Xiao3
1.Department of Stomatology,the Sixth Medical Center of PLA General Hospital,Beijing 100853,China2.Department of Stomatology,the First Hospital of the Southern Theater Command of the Navy,Zhanjiang 524000,China3.Department of Stomatology,the First Medical Center of PLA General Hospital,Beijing 100853,China4.Xinhua Hospital of Tongzhou District,Beijing 101121,China
摘要：Objective Oral diseases exhibit a multifactorial etiology.Plant-derived antimicrobial agents have gained recog-nition in daily personal care because of their eco-friendly,natural,and biodegradable properties.This study aimed to investigate the antibacterial efficacy of Nanyang kiwifruit extract toothpaste against common oral pathogens,in comparison with four commercially available toothpastes,to identify effective strategies for controlling pathogen-ic microorganisms and preventing oral diseases.
Methods Five toothpaste formulations(grape probiotic,white,grape-flavored,peach-flavored,and kiwifruit ex-tract)were prepared as gradient dilutions via two-fold serial dilution.Each dilution series was mixed 1:1 with stan-dardized suspensions of Candida albicans,Staphylococcus aureus,Escherichia coli,or Proteus vulgaris(0.5×106 CFU/ml),followed by overnight incubation at 37℃.Minimum inhibitory concentrations(MICs)were determined spectrophotometrically at OD600.Minimum bactericidal concentrations(MBCs)were subsequently assessed by plate spreading of 100 μl aliquots of the overnight cultures onto LB agar,with bactericidal activity confirmed as a≥99.9%CFU reduction.
Results The kiwifruit extract toothpaste demonstrated comprehensive antibacterial activity against all four tested pathogens,exhibiting broader-spectrum inhibitory effects compared with commercial toothpastes.
Conclusion The Nanyang kiwifruit extract toothpaste demonstrated broad-spectrum antimicrobial activity against both bacterial and fungal pathogens,exhibiting superior efficacy and broader coverage compared with single-tar-get commercial toothpastes.Its multi-pathogen inhibitory capacity addresses a critical limitation of conventional antibacterial toothpastes that primarily target specific microbial subsets,thereby providing greater microbial con-trol for oral ecological balance.
机标关键词：antibacterialcommonoraleffectsextractkiwifruitnanyangpathogens
论文发表日期：2025-06-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 50-59,74 )
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