Circ_0087199 depletion alleviates lps-induced oxidative injury and cell cycle arrest in human periodontal ligament cells via the mir-527/tlr4 axis
Jing Wang1
Na Huo2
Chuan Cai3
Rui Xiao1
1.Department of Stomatology,The First Medical Center of PLA General Hospital,28 Fuxing Road,Haidian District,Beijing,China2.Second author,Department of Stomatology,The First Medical Center of PLA General Hospital,28 Fuxing Road,Haidian District,Beijing,China3.Third author,Department of Stomatology,The First Medical Center of PLA General Hospital,28 Fuxing Road,Haidian District,Beijing,China
摘要：Background Circular RNAs(circRNAs)participate in the development of periodontitis.The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell(PDLC)injury during periodontitis.
Methods PDLCs were treated with lipopolysaccharides(LPS)to establish a periodontitis cell model.Quanti-tative real-time polymerase chain reaction was used to detect the expression of circ_0087199,miR-527,toll-like receptor 4(TLR4).Western blotting assay was performed to assess protein expression.Cell viability,prolifer-ation,apoptosis and inflammation were investigated by cell counting kit-8,EdU assay,flow cytometry and en-zyme-linked immunosorbent assay,respectively.Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit.The interaction between miR-527 and circ_0087199 or TLR4 was con-firmed by a dual-luciferase reporter assay.
Results Circ_0087199 and TLR4 expression levels were significantly increased,while miR-527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS-stimulated PDLCs when compared with con-trols.LPS treatment inhibited cell viability and proliferation but induced cell apoptosis,inflammation and oxida-tive stress,whereas these effects were attenuated after circ_0087199 knockdown.Circ_0087199 bound to miR-527 and regulated LPS-caused PDLC damage by targeting miR-527.Additionally,the overexpression of TLR4,a target gene of miR-527,rescued miR-527 mimic-mediated effects on LPS-treated PDLCs.Further,the regulation of circ_0087199 toward TLR4 involved miR-527.
Conclusion Circ_0087199 knockdown attenuated LPS-induced apoptosis,inflammation and oxidative stress of PDLCs by regulating the miR-527/TLR4 pathway.
机标关键词：humancellsaxiscyclealleviatesarrestcirc_0087199depletion
论文发表日期：2025-12-30
在线出版日期：2026-01-27（本平台首次上网日期，不代表文献的发表时间）
页数：15( 43-57 )
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