Altered expression profile of long non-coding RNAs during heart aging in mice
Xiuxiu Wang1
Bingjie Hua1
Meixi Yu1
Shenzhen Liu1
Wenya Ma1
Fengzhi Ding1
Qi Huang1
Lai Zhang1
Chongwei Bi1
Ye Yuan2
Mengyu Jin1
Tianyi Liu1
Ying Yu1
Benzhi Cai2
Baofeng Yang2
1.Department of Pharmacy at the Second Affiliated Hospital, and Department of Pharmacology at College of Pharmacy (the Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), Harbin Medical University, Harbin 150086, China2.Department of Pharmacy at the Second Affiliated Hospital, and Department of Pharmacology at College of Pharmacy (the Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), Harbin Medical University, Harbin 150086, China;Institute of Clinical Pharmacy, Harbin Medical University, Harbin 150086, China
摘要：Objective: Long noncoding RNAs (lncRNAs) play an important role in regulating the occurrence and development of cardiovascular diseases. However, the role of lncRNAs in heart aging remains poorly understood. The objective of this study was to identify differentially expressed lncRNAs in the heart of aging mice and elucidate the relevant regulatory pathways of cardiac aging.Materials and methods: Echocardiography was used to detect the cardiac function of 18-months (aged) and 3-months (young) old C57BL/6 mice. Microarray analysis was performed to unravel the expression profiles of lncRNAs and mRNAs, and qRT-PCR to verify the highly dysregulated lncRNAs. Results: Our results demonstrated that the heart function in aged mice was impaired relative to young ones. Microarray results showed that 155 lncRNAs were upregulated and 37 were downregulated, and 170 mRNAs were significantly upregulated and 44 were remarkably downregulated in aging hearts. Gene ontology analysis indicated that differentially expressed genes are mainly related to immune function, cell proliferation, copper ion response, and cellular cation homeostasis. KEGG pathway analysis showed that the differentially expressed mRNAs are related to cytokine-cytokine receptor interaction, inflammatory mediator regulation of TRP channels, and the NF-kappa B signaling pathway. Conclusion: These results imply that the differentially expressed lncRNAs may regulate the development of heart aging. This study provides a new perspective on the potential effects and mechanisms of lncRNAs in heart aging.
机标关键词：expressionprofilelongheartrnasmiceagingaltered
论文发表日期：2022-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 109-118 )
英文信息