Cold exposure alters proteomic profiles of the hypothalamus and pituitary in female rats
Xiangyu Bian
Xi Li
Tong Xu
Li Zhang
Yongqiang Zhang
Shuai Wu
Renren Yang
Weiyun Dong
Changjiang Guo
Danfeng Yang
Weina Gao
Tianjin Institute of Environmental and Operational Medicine,Tianjin 300050,China
摘要：Objective:Studies have shown that both short-term and long-term cold exposures disturb the biological process.The aim of the present study is to investigate the effects of intermittent cold exposure on proteomic profiles in the hypothalamus and pituitary of female Sprague-Dawley(SD)rats.Materials and methods:The rats were exposed to -10℃ in a cabin for 4 h per day,and the treatment lasted for 14 days.The comparative label-free LC-MS/MS analysis was performed to investigate the changes of proteomic profiles in the hypothalamus and pituitary.ELISA analysis was used to validate the expression of differential proteins.Results:22 differential proteins in the hypothalamus and 75 differential proteins in the pituitary were identified by the label-free proteomic analysis.Gene ontology annotation and enrichment analysis indicated that cold exposure disrupted protein phosphorylation,filopodium assembly,intracellular protein transport,peripheral nervous system neuron axonogenesis,spinal cord development,Golgi organization,positive regulation of pseudopodium assembly,and cell-cell adhesion.Three proteins(Cdc42,Ptprs,and Setd7)were down-regulated in the cold exposure group.Conclusion:The results indicate that intermittent cold exposure alters the proteomic profiles of hypothalamus and pituitary in female rats.
机标关键词：exposureprofilescoldratsaltersfemalehypothalamuspituitary
论文发表日期：2023-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：12( 114-125 )
英文信息