Characteristics of gut microbiota in anastomotic leakage patients in cold zones post-colorectal cancer surgery:A high-throughput sequencing and propensity-score matching study
Yuliuming Wang1
Yukun Zhang2
Yu Cao3
Weiyuan Zhang1
Ming Liu4
Guiyu Wang1
1.Department of Colorectal Cancer Surgery,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China2.Department of General Surgery,Huashan Hospital,Fudan University,Shanghai 200433,China3.Department of Faculty Surgery No.2,I.M.Sechenov First Moscow State Medical University(Sechenov University),Moscow 119991,Russia4.Department of General Surgery,The fourth Affiliated Hospital of Harbin Medical University,Harbin 150086,China
摘要：Objective:The study aimed to explore the association between gut microbiota and anastomotic leakage(AL)after surgery in colorectal cancer(CRC)patients from a frigid zone,based on high-throughput sequencing.Methods:A total of 98 CRC patients admitted to the Second Affiliated Hospital of Harbin Medical University from July 2018 to February 2019,who met the inclusion criteria,were included.Among these,10 patients were diagnosed as AL.After propensity-score matching of baseline characteristics,10 patients from the anastomotic leakage group(AG)and 10 patients from the normal group(NG)were finally included in this study.Fecal samples were collected,and total DNA was extracted for high-throughput sequencing and bioinformatic analysis.Results:Alpha diversity analysis showed no significant difference between the two groups,while beta diversity analysis revealed significant differences in principal components.Differential microbiota were classified as Proteobacteria at the phylum level(P=0.021).At the genus level,the abundances of Streptococcus(P=0.045),Citrobacter(P=0.008)and Klebsiella(P=0.002)were significantly different between the two groups.LEfSe analysis indicated that these genera contributed most to the differences between the groups.Conclusion:The characteristics of the gut microbiota in the AG and NG were significantly different,and these differences might be associated with AL in CRC patients from frigid zones.
机标关键词：characteristicscancersurgeryscorestudycoldanastomotichigh-throughput
论文发表日期：2024-04-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 120-128 )
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