Comprehensive analysis of cold exposure-associated transcriptional and metabolic changes in the liver
Yuzhu Di1
Zhengchao Wen2
Xiaomin Liu2
Kejiao Zhang2
Xiuyun Shen3
Chunpeng Shi2
Yuqiu Chao4
Xiao Wang2
Shu Wang5
Bo Qu1
Yanan Jiang4
1.Department of Gastroenterology and Hepatology,the Second Affiliated Hospital of Harbin Medical University,Harbin 150081,China2.Department of Pharmacology(National Key Laboratory of Frigid Zone Cardiovascular Diseases,the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education),College of Pharmacy,Harbin Medical University,Harbin 150081,China3.Department of Pharmacology(National Key Laboratory of Frigid Zone Cardiovascular Diseases,the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education),College of Pharmacy,Harbin Medical University,Harbin 150081,China;Translational Medicine Research and Cooperation Center of Northern China,Heilongjiang Academy of Medical Sciences,Harbin 150081,China4.Department of Pharmacology(National Key Laboratory of Frigid Zone Cardiovascular Diseases,the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China,Key Laboratory of Cardiovascular Research,Ministry of Education),College of Pharmacy,Harbin Medical University,Harbin 150081,China;Translational Medicine Research and Cooperation Center of Northern China,Heilongjiang Academy of Medical Sciences,Harbin 150081,China;Department of Oral and Maxillofacial Surgery,the First Affiliated Hospital,Harbin Medical University,Harbin 150081,China5.Department of Cardiology,the First Affiliated Hospital of Harbin Medical University,Harbin 150081,China
摘要：Background:Cold exposure is associated with metabolic alterations.This study aims to investigate the effects and mechanisms of cold exposure on liver metabolism through the integration of transcriptomics and metabolomics.Methods:Liver tissues from mice exposed to cold were subjected to RNA sequencing and liquid chromatography-mass spectrometry(LC-MS)for transcriptomic and metabolomic profiling,respectively.Differentially expressed genes(DEGs)and differentially expressed metabolites(DEMs)were identified.mRNA expression levels were validated by real-time polymerase chain reaction(RT-PCR).Gene ontology(GO),Kyoto encyclopedia of genes and genomes(KEGG),and Reactome enrichment analyses were performed.Finally,transcriptomic and metabolomic data were integrated and analyzed.Results:Cold exposure altered the transcriptomic and metabolomic profiles in the liver in cold exposed mice.Enrichment analyses were of DEGs and DEMs.Enrichment analyses of DEGs and DEMs revealed that DEGs were involved in pathways such as the PI3K-Akt signaling pathway,cytokine-cytokine receptor interaction,and cell adhesion molecules.DEMs were enriched in pathways related to membrane transport,nucleotide metabolism,and the metabolism of cofactors and vitamins.The integration of transcriptomic and metabolomic data identified several pathways potentially associated with cold exposure,such as the PI3K-Akt signaling pathway.Conclusion:Cold exposure alters liver transcriptomic and metabolomic profiles in mice.The integrative analysis of transcriptomic and metabolomic data highlights the complexity of the liver's response to cold exposure and identifies potential targets for further investigation.
机标关键词：metabolicexposureanalysiscoldassociatedchangescomprehensiveliver
论文发表日期：2025-12-30
在线出版日期：2026-01-21（本平台首次上网日期，不代表文献的发表时间）
页数：14( 217-230 )
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