LncRNA-TUG1 as a potential diagnostic biomarker for coronary atherosclerotic heart disease
Xin Wang1
Xuyao Ji2
Siyao Zhang2
Benzhi Cai1
Yu Liu2
1.Department of Pharmacy at The Second Affiliated Hospital,and Department of Pharmacology at College of Pharmacy(the Key Laboratory of Cardiovascular Medicine Research,Ministry of Education);Institute of Clinical Pharmacy,the Heilongjiang Key Laboratory of Drug Research,Harbin Medical University,Harbin 150000,China2.Department of Laboratory medicine,Fourth Affiliated Hospital of Harbin Medical University,Harbin 150001,China
摘要：Objectives:Accumulating evidence suggests that people living in cold regions have a higher risk of developing coronary atherosclerotic heart disease(CHD).Long non-coding RNAs(lncRNAs)have been implicated in the pathogenesis and treatment of a variety of diseases.The present study aimed to investigate the serum level of lncRNA-taurine upregulated gene 1(TUG1)in patients with CHD and assess its potential as a diagnostic biomarker.This study aimes to investigate the serum level of lncRNA-TUG1 in patients with CHD and assess its potential as a diagnostic biomarker.Methods:The Gene Expression Omnibus(GEO)database was employed to identify the potential lncRNAs serving as biomarkers for CHD.To validate lncRNA-TUG1,232 subjects were enrolled in both test and diagnostic cohorts.Serum lncRNA-TUG1 levels were measured by RT-qPCR.The association between lncRNA-TUG1 levels and CHD severity was analyzed using Pearson's correlation test.Diagnostic value was assessed by receiver operating characteristic(ROC)curve analysis and compared with established cardiac biomarkers.Results:LncRNA-TUG1 was identified in the GEO database as a potential biomarker for CHD.Serum lncRNA-TUG1 levels were significantly higher in CHD patients compared with healthy controls and non-CHD patients.CRP levels also differed between CHD and non-CHD groups,while other biomarkers showed no significant differences.ROC curve analysis demonstrated that lncRNA-TUG1 could distinguish CHD from non-CHD patients,with an area under the curve(AUC)of 0.8916,which was higher than that of conventional biomarkers such as cTnI.At a cut-off value of 2.311,the sensitivity and specificity of lncRNA-TUG1 were 61.63%and 97.67%,respectively,surpassing the diagnostic performance of cTnI.Furthermore,lncRNA-TUG1 levels in CHD patients were positively correlated with SYNTAX scores from coronary angiography and increased with the severity of vascular stenosis.Conclusion:Elevated serum lncRNA-TUG1 levels in CHD patients suggest that lncRNA-TUG1 may serve as a novel and valuable diagnostic biomarker for CHD,with potential utility in differentiating CHD from other cardiac diseases.
机标关键词：lncrna-tug1diagnosticheartatheroscleroticbiomarkercoronarydiseasepotential
论文发表日期：2025-12-30
在线出版日期：2026-01-21（本平台首次上网日期，不代表文献的发表时间）
页数：11( 231-241 )
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