An Experimental Study on RBC Count and Serum Potassium Concentration Changes During Compres-sion Transfusion of WBC-removal Whole Blood
Jin-Lian Cheng
Shi-Fan Han
Ya-Qin Li
Yin-Ping Chu
Yu-Mei Sun
Jin-Feng Guo
摘要：Objective: To observe changes in RBC count, changes, RBC morphology, and serum potassium during compressed transfusion of WBC-removal whole blood. <br> Methods: Prepared human WBC-removal whole blood and connected transfusion apparatus with different sizes of intravenous detaining needles ( 18G, 20G, 22G and 24G) . Observed RBC count and serum potassium concentration under different pressures ( 100 mmHg, 200 mmHg, and 300 mmHg) as blood flowed out of the pinhead end of the intravenous detaining needle. Samples ob-tained with the 20G needle were smeared on glass slides, and RBC morphologic changes were ob-served under an oil immersion lens. <br> Results: RBC count and serum potassium changed slightly under different pressures with differ-ent sizes of intravenous detaining needles as blood flowed through the transfusion apparatus. In ad-dition, the observation of blood samples under a common light microscope revealed that coarse-prick, oblong, and spindle cell counts in the visual fields increased gradually as the pressure in-creased. Additionally, a portion of cells had undergone splintering. <br> Conclusions: While applying 18G, 20G, 22G and 24G intravenous detaining needles for blood transfusion under less than 300 mmHg of pressure, no significant RBC count change was found in blood samples in the short term. However, there were significant RBC morphologic changes. The results could offer more basis to ensure the clinical safety of patients undergoing blood transfusion.
机标关键词：Whole Bloodblood transfusionwhole bloodoil immersionspindle cell
资助基金：the Shanxi Science and Technology Development Fund (200233)
论文发表日期：2015-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 88-94 )
英文信息