Influence of Stent Implantation on the Expression of PCNA and Apoptosis in Injured Vascular Smooth Muscle Cells
刘英梅
张旭明
伍卫
王景峰
陈筱潮
1.Department of cardiology, Sun yat - Sen Memorial Hospital. Sun Yat - Sen University, Guangzhou 510120, P R China The grant from Sanitation Department Science Foundation of Guangdong2.Department of cardiology, Sun yat - Sen Memorial Hospital. Sun Yat - Sen University, Guangzhou 510120, P R China The grant from Sanitation Department Science Foundation of Guangdong3.Department of cardiology, Sun yat - Sen Memorial Hospital. Sun Yat - Sen University, Guangzhou 510120, P R China The grant from Sanitation Department Science Foundation of Guangdong4.Department of cardiology, Sun yat - Sen Memorial Hospital. Sun Yat - Sen University, Guangzhou 510120, P R China The grant from Sanitation Department Science Foundation of Guangdong5.Department of cardiology, Sun yat - Sen Memorial Hospital. Sun Yat - Sen University, Guangzhou 510120, P R China The grant from Sanitation Department Science Foundation of Guangdong
摘要：Objectives To evaluate theimpact of stent implantation on proliferation and apop-tosis in injured media vascular smooth muscle cells(VSMC) and to explore the mechanism of restenosisafter stent implantation. Methods Fifty male NewZealand rabbits were randomized into two groups, in-cluding balloon group and stent group. Control groupwas set up. The samples were harvested on 3, 7, 14,28, 56 days after operation and the following investi-proliferating cell nuclear antigen (PCNA) of mediaAnalyzing apoptosis of media VSMC by DNA agarosegel electrophoresis and TUNEL technique. ResultsThe expression of PCNA and apoptosis in stent andballoon groups were markedly increased compared withcreased expression of PCNA in the media VSMC com-pared with balloon group on 3 to 28 days. On day 7,the positive rates of PCNA were 24.36 ± 0. 55 % vsdays, stent group appeared obvious DNA ladder, whileshowed that stent group induced much more significantapoptosis than that of balloon group on 3 to 28 days.The highest rate of apoptosis appeared on day 7:12.42calculating the ratio of the positive rate of PCNA toapoptosis, it showed that on 3 to 28 days, the ratio ofballoon group was higher than that of stent group.There was obvious difference between two groups.Conclusions Stent group induces augmented prolif-eration and much more significant apoptosis of mediaVSMC than that of balloon group. It makes the ratio ofproliferation to apotosis reduced and the severity ofrestenosis relieved after stent implantation.
机标关键词：
分类号：R54(心脏、血管（循环系）疾病)
论文发表日期：2002-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 99-103 )
英文信息