An Experimental Study on the Flexibility of Prevention against Thrombosis Following Mechanical Valve Replacement by tPA Gene Transduction
Shangyi Ji
Jun Ji
Xiaohan Yang
Jiangan Yang
Xiaolei Wang
Wenping Ling
Yuping Zhang
摘要：Objectives Use a gene suture immersed recombinant tissue-type plasminogen activator (r-tPA)expression plasmid to transduce myocardia to prevent the thrombosis after mechanical tricuspid valve replacement in pigs. Methods A r-tPA gene plasmid was constructed and conjugated to a novel cationic phosphonolipid and a r-tPA gene suture was made. Eighteen pigs were selected and divided into two groups at randomization. There were 9 pigs in the experimental group and 9 in the control group, all the 18 pigs' tricuspids were replaced with mechanical valves. The gene threads were sutured into the right ventficular walls near mechanical valves and an ultrasound was used on the surfaces of the right ventricular walls for the gene transfer in the experimental group. Coagulative function, D-dimer level of the blood and the thrombosis on the surfaces of the valves were observed. Results r-tPA gene plasmid was successfully con-strutted and r-tPA protein was expressed in the ventricular cells around the gene sutures. D-dimer reached its peak level the experiment in experimental group. The thromboses around the valves were found in all the control group (100%)but only 1 (11.11%) case in experimental group. There were no changes in prothrombin time pre and post operation in two groups. Conclusions Using gene suture immersed r-tPA expression plasmid to transduce myocardia might be a best substitution for life long anti-coagulation therapy for the patients, who underwent operation.
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分类号：R5(内科学)
论文发表日期：2008-05-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 134-138 )
英文信息