Morphine Preconditioning-induced Endothelial Protection against in vitro Simulated Ischemia-reperfusion Injury
Long-yun PENG
Ai-zhen YAN
Xiu-ren GAO
Gui-fu WU
Wei-yi MAI
Hong MA
Zhi-yi ZUO
摘要：Objectives To determine whether morphine induced a preconditioning effect in endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were subjected to oxygen-glucose deprivation (OGD) to simulate ischemia in vitro. Cell viability was evaluated by the 3-(4, 5-dimethylthiazol -2-yl) -2, 5-diphenyltetrazolium bromide assay. Results OGD time-dependently decreased the HUVEC viability. Morphine pretreatment applied 5 min or 24 hr before a 20-hr OGD dose-dependently attenuated the OGD-reduced cell viability. The ECS0 for this effect was 1.84 and 0. 26μM, respectively, for morphine applied 5 min or 24 hr before the OGD. Conclusion morphine induces ischemic tol-erance in endothelial cells.
机标关键词：
分类号：R5(内科学)
论文发表日期：2009-04-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 80-84 )
英文信息