Germ cell-specific deletion of Pex3 reveals essential roles of PEX3-dependent peroxisomes in spermiogenesis
Yejin Yao1
Baolu Shi2
Xiangzheng Zhang1
Xin Wang1
Shuangyue Li1
Ying Yao1
Yueshuai Guo1
Dingdong Chen1
Bing Wang1
Yan Yuan1
Jiahao Sha3
Xuejiang Guo1
1.State Key Laboratory of Reproductive Medicine and Offspring Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.Reproductive and Genetic Branch,the First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui 230001,China3.State Key Laboratory of Reproductive Medicine and Offspring Health,Women's Hospital of Nanjing Medical University,Nanjing Maternity and Child Health Care Hospital,Nanjing Medical University,Nanjing,Jiangsu 211166,China
摘要：Peroxisomes are organelles enclosed by a single membrane and are present in various species.The abruption of peroxisomes is correlated with peroxisome biogenesis disorders and single peroxisomal enzyme deficiencies that induce diverse diseases in different organs.However,little is known about the protein compositions and corresponding roles of heterogeneous peroxisomes in various organs.Through transcriptomic and proteomic analyses,we observed heterogenous peroxisomal components among different organs,as well as between testicular somatic cells and different developmental stages of germ cells.As Pex3 is expressed in both germ cells and Sertoli cells,we generated Pex3 germ cell-and Sertoli cell-specific knockout mice.While Pex3 deletion in Sertoli cells did not affect spermatogenesis,the deletion in germ cells resulted in male sterility,manifested as the destruction of intercellular bridges between spermatids and the formation of multinucleated giant cells.Proteomic analysis of the Pex3-deleted spermatids revealed defective expressions of peroxisomal proteins and spermiogenesis-related proteins.These findings provide new insights that PEX3-dependent peroxisomes are essential for germ cells undergoing spermiogenesis,but not for Sertoli cells.
机标关键词：germcell-specificdeletionessentialperoxisomespex3-dependentrevealsroles
分类号：R321.1(人体形态学)
论文发表日期：2024-01-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：14( 中插2,24-36 )
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