circPVT1 promotes silica-induced epithelial-mesenchymal transition by modulating the miR-497-5p/TCF3 axis
Siyun Zhou1
Yan Li2
Wenqing Sun1
Dongyu Ma1
Yi Liu3
Demin Cheng1
Guanru Li1
Chunhui Ni1
1.Department of Occupational Medical and Environmental Health,Key Laboratory of Modern Toxicology of Ministry of Education,Center for Global Health,School of Public Health,Nanjing Medical University,Nanjing,Jiangsu 211166,China2.Biomedical Publications Center,Nanjing Medical University,Nanjing,Jiangsu 211166,China3.Gusu School,Nanjing Medical University,Nanjing,Jiangsu 211166,China
摘要：Epithelial-mesenchymal transition(EMT)is a vital pathological feature of silica-induced pulmonary fibrosis.However,whether circRNA is involved in the process remains unclear.The present study aimed to investigate the role of circPVT1 in the silica-induced EMT and the underlying mechanisms.We found that an elevated expression of circPVT1 promoted EMT and enhanced the migratory capacity of silica-treated epithelial cells.The isolation of cytoplasmic and nuclear separation assay showed that circPVT1 was predominantly expressed in the cytoplasm.RNA immunoprecipitation assay and RNA pull-down experiment indicated that cytoplasmic-localized circPVT1 was capable of binding to miR-497-5p.Furthermore,we found that miR-497-5p attenuated the silica-induced EMT process by targeting transcription factor 3(TCF3),an E-cadherin transcriptional repressor,in the silica-treated epithelial cells.Collectively,these results reveal a novel role of the circPVT1/miR-497-5p/TCF3 axis in the silica-induced EMT process in lung epithelial cells.Once validated,this finding may provide a potential theoretical basis for the development of interventions and treatments for pulmonary fibrosis.
机标关键词：axiscircpvt1epithelialmesenchymalmodulatingpromotessilica-inducedtransition
分类号：R135.2(劳动卫生)
论文发表日期：2024-03-30
在线出版日期：2026-03-31（本平台首次上网日期，不代表文献的发表时间）
页数：13( 中插4,163-174 )
英文信息